چاپ صفحه |  صفحه نخست سامانه |  نویسندگان |  اطلاعات تفضیلی |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده | نفر چندم مقاله |
|---|---|
| نرجس صدیقی | اول |
| مهدی طالبی | دوم |
| عنوان | متن |
|---|---|
| کلمات کلیدی | Bone Marrow, Mesenchymal Stem Cell, Extracellular Vesicles, Exosome, Hypoxia |
| خلاصه مقاله | Objective: MSC-derived exosomes have therapeutic potential by delivering anti-tumor agents and regulatory RNAs. Hypoxia can mediate the tumor microenvironment and is involved in the metastasis and progression of tumor cells. Previous research has indicated that hypoxic conditions can provide a better condition for MSC-derived exosomes and aid in their therapeutic potential. This study analyzed GEO data to explore how hypoxia affects mRNA expression in BMMSC-exosomes. Materials and Methods: At first, GEO was used to examine the RNAseq data of BMMSC-derived exosomes in hypoxia and normoxia conditions to identify all DEGs linked with hypoxia conditions. Thereafter, based on significant genes(p-value ≤ 0.05), a Protein-protein interaction(PPI) network was constructed with STRING at the Cytoscape software for identifying hub genes. Ultimately, enrichment analysis, including Gene Ontology(GO), was used to explore the related biological processes, molecular functions, cellular components, and KEGG pathway analysis for DEGs. Results: From 15990 genes obtained from GEO-RNAseq for MSC-derived exosome contents in hypoxic conditions rather than normoxic, 967 DEGs were identified, of which 589 genes were up-regulated, and 338 were down-regulated. According to PPI networks, we identified 767 nodes, based on their degree and interaction; 185 of them have more than 10 interactions and node-degree, including CDCA8, PLK1, CCNB1, AURKA, CDC20, BIRC5, BUB1B, FEN1, and TRIM28. Furthermore, the enrichment analysis showed DEGs have the strongest significance in the Assembly of Checkpoint Signaling, Cell cycle, Golgi and mitochondria cellular components, VEGF, Pentose phosphate, FoxO and P53 signalling pathways, and so on. Conclusion: Our study identified DEGs, hub genes, and key signalling pathways that may be involved in hypoxic conditions. These findings contribute to a better understanding of cellular survival mechanisms under hypoxia and may aid in identifying potential prognostic and diagnostic biomarkers. Further investigations are warranted to explore the effects of these exosomes on cancer cell lines. |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| AbstractBook.pdf | 1404/08/22 | 270238 | دانلود |
| New Microsoft Word Document.pdf | 1404/10/06 | 162079 | دانلود |
