چاپ صفحه |  صفحه نخست سامانه |  نویسندگان |  اطلاعات تفضیلی |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده ثبت کننده مقاله | امین خامنه |
| مرحله جاری مقاله | تایید نهایی |
| دانشکده/مرکز مربوطه | کمیته تحقیقات دانشجویی |
| کد مقاله | 88918 |
| عنوان فارسی مقاله | اثرات محافظتی اگزوزومهای مشتق از سلولهای بنیادی مزانشیمی بر عملکرد تخمدان در مدل ناباروری ناشی از شیمیدرمانی |
| عنوان لاتین مقاله | Protective Effects of Mesenchymal Stem Cell-Derived Exosomes on Ovarian Function in a Chemotherapy-Induced Infertility Model |
| نوع ارائه | پوستر |
| عنوان کنگره / همایش | بیستمین کنگره بین المللی بیماری های زنان و زایمان |
| نوع کنگره / همایش | بین المللی |
| کشور محل برگزاری کنگره/ همایش | Iran (Islamic Republic) |
| شهر محل برگزاری کنگره/ همایش | تهران |
| سال انتشار/ ارائه شمسی | 1404 |
| سال انتشار/ارائه میلادی | 2025 |
| تاریخ شمسی شروع و خاتمه کنگره/همایش | 1404/03/21 الی 1404/03/24 |
| آدرس لینک مقاله/ همایش در شبکه اینترنت | https://naigo2025.ir/ |
| آدرس علمی (Affiliation) نویسنده متقاضی | Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran. |
| نویسنده | نفر چندم مقاله |
|---|---|
| امین خامنه | اول |
| عنوان | متن |
|---|---|
| خلاصه مقاله | Background and Aim : Chemotherapy-induced ovarian damage can cause infertility through oxidative stress and follicular depletion. Mesenchymal stem cell-derived exosomes show potential for tissue repair. This study evaluated the effects of mesenchymal stem cell-derived exosomes on ovarian function restoration in a chemotherapy-induced infertility model by assessing hormonal levels, oxidative stress, histopathological changes, and apoptotic markers. Methods : Adult female Wistar rats were randomly assigned to four groups: control, chemotherapy (cyclophosphamide, 100 mg/kg, single dose), mesenchymal stem cell-derived exosome treatment, and combination (exosomes + chemotherapy). Bone marrow-derived mesenchymal stem cells (BMSCs) were isolated, cultured, and characterized by flow cytometry. Exosomes were isolated from BMSCs using ultracentrifugation. Histopathological analysis was performed to confirm cyclophosphamide-induced ovarian damage before initiating treatment. A dose of 10 μg exosomes per rat was administered intraperitoneally for 5 consecutive days, starting 5 days post-chemotherapy. Hormonal levels, oxidative stress, apoptosis, and histopathological changes were assessed. Results : Histopathological analysis confirmed ovarian damage in the chemotherapy group, with follicular depletion and stromal degeneration. Exosome treatment improved ovarian structure, increasing healthy follicles. Hormonal analysis showed decreased estrogen and progesterone and increased FSH and LH in the chemotherapy group (p < 0.05). Exosome treatment partially restored hormonal balance (p < 0.05). Oxidative stress markers revealed elevated MDA and reduced SOD, GPx, and CAT in the chemotherapy group (p < 0.05). Exosome therapy decreased MDA and enhanced antioxidant enzyme activity (p < 0.05). Apoptosis analysis showed increased BAX and decreased BCL-2 in the chemotherapy group (p < 0.05), while exosome treatment reversed these changes (p < 0.05). Conclusion : Mesenchymal stem cell-derived exosomes effectively mitigate chemotherapyinduced ovarian damage by restoring hormonal balance, reducing oxidative stress, and suppressing apoptosis. This therapy shows promise as a non-invasive strategy for preserving ovarian function and fertility during chemotherapy. |
| کلمات کلیدی | Ovarian function, Chemotherapy-induced infertility, Exosomes, Mesenchymal stem cells, Fertility preservation, Oxidative stress |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| Abstract.docx | 1404/08/18 | 135079 | دانلود |
| Certificate.pdf | 1404/08/18 | 143025 | دانلود |
