چاپ صفحه |  صفحه نخست سامانه |  نویسندگان |  اطلاعات تفضیلی |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده ثبت کننده مقاله | امین خامنه |
| مرحله جاری مقاله | تایید نهایی |
| دانشکده/مرکز مربوطه | کمیته تحقیقات دانشجویی |
| کد مقاله | 88917 |
| عنوان فارسی مقاله | بررسی اثر همافزایی سلولهای بنیادی مزانشیمی مشتق از مغز استخوان و ملاتونین بر بهبود ناباروری ناشی از استرس اکسیداتیو در مدل موش صحرایی |
| عنوان لاتین مقاله | Investigating the Synergistic Effect of Bone Marrow-Derived Mesenchymal Stem Cells and Melatonin on Improving Infertility Caused by Oxidative Stress in a Rat Model |
| نوع ارائه | پوستر |
| عنوان کنگره / همایش | بیستمین کنگره بین المللی بیماری های زنان و زایمان |
| نوع کنگره / همایش | بین المللی |
| کشور محل برگزاری کنگره/ همایش | Iran (Islamic Republic) |
| شهر محل برگزاری کنگره/ همایش | تهران |
| سال انتشار/ ارائه شمسی | 1404 |
| سال انتشار/ارائه میلادی | 2025 |
| تاریخ شمسی شروع و خاتمه کنگره/همایش | 1404/03/21 الی 1404/03/24 |
| آدرس لینک مقاله/ همایش در شبکه اینترنت | https://naigo2025.ir/ |
| آدرس علمی (Affiliation) نویسنده متقاضی | Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran. |
| نویسنده | نفر چندم مقاله |
|---|---|
| امین خامنه | اول |
| عنوان | متن |
|---|---|
| کلمات کلیدی | Infertility, Mesenchymal Stem Cells, Oxidative Stress ,Melatonin, Ovarian Function |
| خلاصه مقاله | Background and Aim : Infertility caused by oxidative stress impairs ovarian function, leading to reduced fertility. Mesenchymal stem cells (MSCs) and melatonin, both with regenerative and antioxidant properties, have shown potential in treating infertility. This study evaluated the combined effect of bone marrow-derived MSCs and melatonin in a rat model. Specifically, it assessed the impact on oxidative stress, hormonal levels, histopathology, and apoptosis markers, with the goal of improving fertility in oxidative stress-induced infertility. Methods : Adult female Wistar rats (200-250g) were used. Oxidative stress was induced by a single intraperitoneal injection of 100 mg/kg cyclophosphamide (CP). After 5 days, following confirmation of oxidative stress induction by ovarian tissue analysis, treatment with melatonin (10 mg/kg daily, IP) and bone marrow-derived mesenchymal stem cells (BMSCs, 1 × 10⁶ cells, administered once IP) began. The rats were divided into four groups: 1) Control, 2) Melatonin, 3) BMSCs, and 4) Combination group. BMSCs were isolated, cultured, and characterized. Melatonin was administered for 14 days. Ovarian function was assessed by measuring hormonal levels, oxidative stress markers, and histopathological evaluation. Apoptosis was assessed by BAX and BCL-2 expression. Results : Treatment with melatonin and BMSCs improved ovarian function in cyclophosphamideinduced oxidative stress infertility in rats. Hormonal levels (FSH, LH, estradiol, AMH) increased, with the combination group showing the best recovery. Oxidative stress markers changed significantly: MDA decreased, while SOD, CAT, and GPx increased, especially in the combination group. Histopathology showed less ovarian damage, fewer apoptotic signs, and better tissue preservation. Apoptotic markers expression were regulated, BAX expression decreased, while BCL-2 increased in treated groups. Conclusion : The combination of melatonin and BMSCs effectively protected against oxidative stress-induced infertility by reducing ovarian damage, restoring hormone levels, decreasing apoptosis, and preserving tissue structure, showing superior therapeutic potential. |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| Abstract.docx | 1404/08/18 | 140479 | دانلود |
| Certificate.pdf | 1404/08/18 | 145254 | دانلود |
