چاپ صفحه |  صفحه نخست سامانه |  نویسندگان |  اطلاعات تفضیلی |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده ثبت کننده مقاله | لیلا واحدی |
| مرحله جاری مقاله | تایید نهایی |
| دانشکده/مرکز مربوطه | مرکز مدیریت و پیشگیری از مصدومیتهای حوادث ترافیکی |
| کد مقاله | 88883 |
| عنوان فارسی مقاله | شناسایی مولکولی بورخولدریا سپاسیه از بیماران فیبروز کیستیک در شمال غرب ایران: یک یادداشت پژوهشی |
| عنوان لاتین مقاله | Molecular identification of Burkholderia cepacia from cystic fibrosis patients in northwestern Iran: a research note |
| نوع ارائه | پوستر |
| عنوان کنگره / همایش | هجدهمین کنگره بین المللی میکروب شناسی بالینی استاد البرزی |
| نوع کنگره / همایش | بین المللی |
| کشور محل برگزاری کنگره/ همایش | Iran (Islamic Republic) |
| شهر محل برگزاری کنگره/ همایش | شیراز |
| سال انتشار/ ارائه شمسی | 1404 |
| سال انتشار/ارائه میلادی | 2025 |
| تاریخ شمسی شروع و خاتمه کنگره/همایش | 1404/07/29 الی 1404/08/01 |
| آدرس لینک مقاله/ همایش در شبکه اینترنت | https://congress.alborzicmrc.ir/ |
| آدرس علمی (Affiliation) نویسنده متقاضی | Road Traffic Injury Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. |
| نویسنده | نفر چندم مقاله |
|---|---|
| امیر حسین جعفری روحی | اول |
| لیلا واحدی | دوم |
| عنوان | متن |
|---|---|
| خلاصه مقاله | Introduction The Burkholderia c complex (BCC) is a major cause of secondary infections in patients with cystic fibrosis (CF). Despite advances in global surveillance, reliably detecting of BCC remains challenging in terms of morbidity and mortality worldwide. Materials From September 2023 to June 2024, periodic three-month assessments wert ducted in northwestern Iran to monitor secondary infections in patients with cystic fibrosis. Sputum samples were collected from cach patient and subjected to conventional culture on selective media, followed by biochemical tests for preliminary identification. Concurrently, molecular detection was performed using a polymerase chain reaction (PCR) targeting specific Burkholderia cepacia complex (BCC) genes, enabling precise and rapid identification of B. cepacia. The study also evaluated the potential application of an enzyme-linked immunosorbent assay (ELISA)-based approach using native bacterial strains to develop a rapid and specific diagnostic assay. This study aimed to compare the efficiency, specificity, and reliability of conventional versus molecular diagnostic methods in this patient population by combining culture, biochemical, PCR, and ELISA evaluations. Results B. cepacia was not detected in any of the 100 samples using culture and biochemical methods. However, PCR identified five positive samples. These results suggest that conventional culture and commercial biochemical systems may be unreliable for detecting B. cepaciae, whereas molecular methods are highly specific and accurate. Conclusion Molecular detection, particularly PCR, is a precise method for identifying B. cepacia. Additionally, developing ELISA assays using native species could improve the speed, specificity, and efficiency of BCC detection in a clinical setting. |
| کلمات کلیدی | Burkholderia cepacia complex, Cystic Fibrosis, PCR, ELISA |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| Abstract.jpg | 1404/08/15 | 1426458 | دانلود |
| Certificat.jpg | 1404/08/15 | 402666 | دانلود |
| Pay.Alborz.docx | 1404/08/15 | 185201 | دانلود |
