چاپ صفحه |  صفحه نخست سامانه |  نویسندگان |  اطلاعات تفضیلی |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده ثبت کننده مقاله | سپیده زنونی واحد |
| مرحله جاری مقاله | تایید نهایی |
| دانشکده/مرکز مربوطه | مرکز تحقیقات کلیه |
| کد مقاله | 88579 |
| عنوان فارسی مقاله | Pre-Transplant Mixed Lymphocyte Reaction (MLR) and Its Association With Kidney Allograft Function and Donor-Specific Antibody Development: A Prospective Study |
| عنوان لاتین مقاله | Pre-Transplant Mixed Lymphocyte Reaction (MLR) and Its Association With Kidney Allograft Function and Donor-Specific Antibody Development: A Prospective Study |
| نوع ارائه | سخنرانی |
| عنوان کنگره / همایش | Eurasian-Iranian Organ Transplant Congress |
| نوع کنگره / همایش | بین المللی |
| کشور محل برگزاری کنگره/ همایش | Iran (Islamic Republic) |
| شهر محل برگزاری کنگره/ همایش | Shiraz |
| سال انتشار/ ارائه شمسی | 1404 |
| سال انتشار/ارائه میلادی | 2025 |
| تاریخ شمسی شروع و خاتمه کنگره/همایش | 1404/02/23 الی 1404/02/25 |
| آدرس لینک مقاله/ همایش در شبکه اینترنت | https://shiraztransplantcongress.sums.ac.ir/en/?time=1759295910 |
| آدرس علمی (Affiliation) نویسنده متقاضی | Kidney Research Center, Tabriz University of Medical Sciences, Tabriz Iran |
| نویسنده | نفر چندم مقاله |
|---|---|
| سپیده زنونی واحد | اول |
| محمد رضا اردلان | سوم |
| علیرضا مردمی | دوم |
| سیده مینا حجازیان | چهارم |
| عنوان | متن |
|---|---|
| خلاصه مقاله | Considering the importance of mixed lymphocyte reaction (MLR) in determining immunological compatibility between donors and recipients before transplantation, this prospective study aimed to evaluate CFSE-MLR, kidney function, and donor-specific antibodies (DSA) to improve transplant outcomes. The study investigated MLR between donors and recipients and its effects on graft function. This study included 40 donor-recipient pairs meeting cross-match criteria for transplantation. Peripheral blood (5 mL) was collected from donors and recipients, and peripheral blood mononuclear cells (PBMCs) were isolated. Cell viability was determined using trypan blue staining. Recipient PBMCs (responder cells) were labeled with the cytoplasmic fluorescent dye CFSE and analyzed via flow cytometry. Transplanted kidney function was assessed by measuring serum creatinine and BUN levels, correlating these with MLR cellular proliferation. The relationship between pre-transplant MLR proliferation rates and donor-specific antibody (DSA) titers was examined, alongside correlations between MLR results, DSA, and PRA% with other pre-transplant tests. The percentage of proliferating cells increased significantly on days 3 and 5 compared to day 0. A significant positive correlation was observed between day 5 cellular proliferation and PRA Class II levels. ELISA results showed no significant correlation between pre-transplant cellular proliferation and serum DSA titers at six months. However, a positive and significant correlation was found between day 5 MLR proliferation and serum creatinine levels six months post-transplant. This study suggests that the CFSE-MLR method is a safer and more efficient alternative to conventional MLR, offering valuable diagnostic insights and strong correlations with transplant success. |
| کلمات کلیدی |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| MLR-2025-abstract.pdf | 1404/07/15 | 283335 | دانلود |
| MLR-2025-certificate.pdf | 1404/07/15 | 7269182 | دانلود |
| Abstract Book Cover-2025.pdf | 1404/07/15 | 1408517 | دانلود |
