چاپ صفحه |  صفحه نخست سامانه |  نویسندگان |  اطلاعات تفضیلی |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده ثبت کننده مقاله | سید امیر مرادیان |
| مرحله جاری مقاله | تایید نهایی |
| دانشکده/مرکز مربوطه | کمیته تحقیقات دانشجویی |
| کد مقاله | 88378 |
| عنوان فارسی مقاله | ارزیابی آزمایشگاهی بافت بیضه کشت شده در محیط حاوی پلاسمای غنی از فاکتورهای رشد (PRGF) و جایگزینی سرم KnockOut (KSR) |
| عنوان لاتین مقاله | In Vitro Assessment of Testicular Tissue Cultured in a Medium Containing Plasma Rich in Growth Factors (PRGF) and KnockOut Serum Replacement (KSR) |
| نوع ارائه | پوستر |
| عنوان کنگره / همایش | 21st International Congress of Stem Cell Biology & Technology and the 26th International Congress of Reproductive Biomedicine |
| نوع کنگره / همایش | بین المللی |
| کشور محل برگزاری کنگره/ همایش | Iran (Islamic Republic) |
| شهر محل برگزاری کنگره/ همایش | تهران |
| سال انتشار/ ارائه شمسی | 1404 |
| سال انتشار/ارائه میلادی | 2025 |
| تاریخ شمسی شروع و خاتمه کنگره/همایش | 1404/06/12 الی 1404/06/14 |
| آدرس لینک مقاله/ همایش در شبکه اینترنت | https://royancongress.com/AbstractBook2025.pdf |
| آدرس علمی (Affiliation) نویسنده متقاضی | Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran |
| نویسنده | نفر چندم مقاله |
|---|---|
| سید امیر مرادیان | اول |
| عنوان | متن |
|---|---|
| خلاصه مقاله | Introduction: Cancer therapies often result in infertility, particularly in prepubescent males. This research evaluates a novel testicular tissue culture medium supplemented with both plasma rich in growth factors (PRGF) and KnockOut serum replacement (KSR) for its potential to support in vitro spermatogenesis. Method: Using five-day-old male NMRI mice, we compared the outcomes of culturing testicular tissue in a medium containing 5% KSR and 5% PRGF versus a control medium with 10% KSR alone. Results: After 42 days of culture, the group receiving 5%KSR+5%PRGF exhibited notable degeneration of the peripheral seminiferous tubules relative to the 10% KSR group. Analysis of gene expression demonstrated significantly reduced levels of spermatogenesis-related markers (Plzf, Tekt1, Tnp1) and the proliferation marker Ki67, alongside increased expression of the pro-apoptotic marker Bax. Quantitative immunofluorescence results corroborated these findings, showing marked declines in spermatogonial stem cells (PLZF), spermatocytes (SYCP-3), and proliferating cells (Ki67), with the complete absence of the post-meiotic marker ACRBP in the 5%KSR+5%PRGF group. Furthermore, the fluorescence intensity for Bax was elevated, while that for Bcl-2 was significantly diminished, compared to the 10% KSR group. Conclusion: In summary, the combination of 5% KSR and 5% PRGF failed to support complete in vitro spermatogenesis and instead promoted cellular degeneration and apoptosis. The reduced expression of key germ cell and proliferation markers, alongside increased apoptotic signaling, suggests that this medium disrupts the microenvironment required for germ cell development. These results suggest that the KSR and PRGF combination, in the tested ratio, is not an appropriate formulation for maintaining or promoting spermatogenic progression in vitro. |
| کلمات کلیدی | In vitro spermatogenesis, PRGF, KSR, spermatogonial stem cells, organ culture |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| Untitledcccc.jpg | 1404/06/16 | 191337 | دانلود |
| EPoster-Amir moradian-2025.jpg | 1404/06/16 | 2852148 | دانلود |
| 22.pdf | 1404/06/24 | 110651 | دانلود |
