چاپ صفحه |  صفحه نخست سامانه |  نویسندگان |  اطلاعات تفضیلی |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده ثبت کننده مقاله | نیلوفر پوراکبر |
| مرحله جاری مقاله | تایید نهایی |
| دانشکده/مرکز مربوطه | کمیته تحقیقات دانشجویی |
| کد مقاله | 88113 |
| عنوان فارسی مقاله | بررسی سطح پلاسمایی آنزیم PARP-2 در بیماران با شکست لانه گزینی مکرر و زنان بارور سالم |
| عنوان لاتین مقاله | Evaluating poly (ADP-ribose) polymerase-2 plasma levels in repeated implantation failure and fertile women |
| نوع ارائه | سخنرانی |
| عنوان کنگره / همایش | بیست و پنجمین کنگره ملی و یازدهمین کنگره بین المللی سالیانه پژوهش و فناوری دانشجویان علوم پزشکی کشور - ارومیه |
| نوع کنگره / همایش | بین المللی |
| کشور محل برگزاری کنگره/ همایش | |
| شهر محل برگزاری کنگره/ همایش | ارومیه |
| سال انتشار/ ارائه شمسی | 1403 |
| سال انتشار/ارائه میلادی | 2024 |
| تاریخ شمسی شروع و خاتمه کنگره/همایش | 1403/06/15 الی 1403/06/17 |
| آدرس لینک مقاله/ همایش در شبکه اینترنت | https://ibj.pasteur.ac.ir/article-1-4402-en.pdf |
| آدرس علمی (Affiliation) نویسنده متقاضی | Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran |
| نویسنده | نفر چندم مقاله |
|---|---|
| نیلوفر پوراکبر | اول |
| مهتا پاشاپور | دوم |
| سید عماد سید غلامی شربیانی | سوم |
| امیررضا ناصری | چهارم |
| عنوان | متن |
|---|---|
| خلاصه مقاله | Successful embryo implantation requires both well endometrial receptivity and a highquality embryo. Recurrent implantation failure (RIF) refers to the implantation failure of at least 3 healthy embryos following in vitro fertilization (IVF) in a woman under the age of 40 years. Maternal immune system’s inadequacy is convicted for implantation failure in RIF patients. During the implantation window, high division rate of undifferentiated stem cells of epiblast, exposes them to DNA damage. Poly (ADP-ribosyl) ation (PARylation), prevents blastocyst apoptosis through autophagy induction. Poly (ADP-ribose) polymerase-2 (PARP-2), the major enzyme responsible for PARylation, plays an essential role in DNA repair pathways during early post-implantation development. Animal studies have approved its upregulation throughout the endometrial receptivity stages at basal levels of endometrium. This study aims to evaluate PARP- 2 levels in RIF patients compared to fertile women. In this case-control study, we investigated the expression levels of PARP-2 in peripheral blood samples of RIF patients compared to healthy pregnant women. 24 RIF patients, whose husbands’ semen analysis and DNA fragmentation test results were normal, and 24 individuals as a control group, from gynecology department of Tabriz Valiasr Hospital were enrolled in the study. 10 ml of blood sample was taken from individuals at the follicular phase of their menstrual cycle and the serum levels of PARP-2 were assessed by Human PARP2 ELISA kit. To determine the distribution of Potential confounding variables (age, BMI, cigarette and alcohol addiction), Shapiro-Will test was performed that showed normal distribution. The mean level of PARP-2 and standard deviation were reported. An independentsamples t-test was conducted to compare the mean level between the groups, using SPSS Statistics version 23.0. The mean age was 33.64 ± 3.0 in RIF patients and 32.62 ± 4.01 in control group (p value = 0.98). A significant reduction in PARP-2 serum levels was observed in RIF patients (7.72 ± 4.41 ng/ml) compared with controls (11.06 ± 5.41 ng/ml). (p value = 0.023) This result suggests that PARP-2 probably takes part in cellular events of implantation in human uterine as explored in animal studies to affect endometrial receptivity, embryo development and blastocyst attachment. Additional studies can contribute to more comprehensive understanding of its underlying mechanisms and develop it as a biomarker of uterine receptivity and stromal cell decidualization. |
| کلمات کلیدی | Recurrent implantation failure, PARP-2, Embryo implantation, PARylation, Infertility |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| arcims25.jpg | 1404/05/07 | 549867 | دانلود |
| screen.png | 1404/05/07 | 2564730 | دانلود |
