چاپ صفحه |  صفحه نخست سامانه |  نویسندگان |  اطلاعات تفضیلی |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده | نفر چندم مقاله |
|---|---|
| یلدا رهبرسعادت | اول |
| سپیده زنونی واحد | چهارم |
| محمد رضا اردلان | پنجم |
| عنوان | متن |
|---|---|
| کلمات کلیدی | Bioinformatics, Felzartamab, Kidney transplant |
| خلاصه مقاله | Felzartamab, a CD38 monoclonal antibody, suppresses antibody-mediated rejection (ABMR) in kidney transplant patients but often leads to recurrence post-treatment. It reduces ABMR molecular activity, primarily targeting interferon gamma-inducible and NK cell transcripts, with minimal effect on endothelial transcripts. Suppression is incomplete in severe cases, and molecular recurrence is common by week 52. However, felzartamab shows parenchymal benefits, slowing kidney injury progression beyond treatment, suggesting potential long-term protective effects. To determine the molecular effects of 6 months of felzartamab treatment, genome-wide microarray analysis was performed on trial biopsies, comparing pre-treatment, end-of-treatment (week 24), and post-treatment (week 52) samples from 10 felzartamab and 10 placebo patients. Data no. GSE275824 were obtained from the GEO database, and the expression dataset was downloaded from pre-treatment, end-of-treatment (week 24), and post-treatment (week 52) samples. The data were analyzed with the GEO2R analysis program. As a result, differentially expressed genes that play roles were excluded. Gene ontology and pathway analysis of these genes were carried out with DAVID software. For the analysis of prominent genes, STRING software was used. In the validation of hub genes, genes were determined using the mcode and cytohubba attachments of the CYTOSCAPE software. We investigated significant downregulated DEGs (1031 baseline, 592 24week and 717 52 weeks down-regulated) and upregulated DEGs (900 baseline, 653 24week and 1428 52 weeks down-regulated). In gene ontology, it was observed that genes were collected in endoplasmic reticulum membrane, membrane, RNA polymerase II cis-regulatory region sequence-specific DNA binding, Golgi membrane, regulation of transcription by RNA polymerase II, DNA-binding transcription factor activity, RNA polymerase II-specific, plasma membrane, adaptive immune response, extracellular region, collagen-containing extracellular matrix. Based on the Cytoscape analysis, HLA-A, HLA-B, HLA-C, HLA-E, HLA-F, PPAPDC1A, PPAPDC1B, UQCRH, UQCRC2, COX5A, CYP4A22, RPL30, RPS20 and HIST1H3E genes were defined as hub genes. The findings suggest that felzartamab modulates critical pathways, providing insights into its molecular effects. Understanding these mechanisms may help identify biomarkers for treatment response and guide the development of alternative strategies to improve long-term kidney transplant outcomes. |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| Dr. Yalda Rahbar.pdf | 1404/05/06 | 7267517 | دانلود |
| r_6_250301031200.jpg | 1404/05/06 | 9460251 | دانلود |
| Rahbar Saadat-Abstract.pdf | 1404/07/15 | 8515 | دانلود |
