چاپ صفحه |  صفحه نخست سامانه |  نویسندگان |  اطلاعات تفضیلی |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده ثبت کننده مقاله | صفر فرج نیا |
| مرحله جاری مقاله | تایید نهایی |
| دانشکده/مرکز مربوطه | مرکز تحقیقات کاربردی دارویی |
| کد مقاله | 87327 |
| عنوان فارسی مقاله | بررسی میزان بیان و محلولیت پپتید Teriparatide در فیوژن با Ssp DnaB mini-intein |
| عنوان لاتین مقاله | Expression and solubility of Teriparatide peptide in fusion of Ssp DnaB mini-intein in E. coli |
| نوع ارائه | پوستر |
| عنوان کنگره / همایش | پنجمین کنفرانس علوم پروتئینی و پپتیدی ایران |
| نوع کنگره / همایش | ملی |
| کشور محل برگزاری کنگره/ همایش | Iran (Islamic Republic) |
| شهر محل برگزاری کنگره/ همایش | تهران |
| سال انتشار/ ارائه شمسی | 1403 |
| سال انتشار/ارائه میلادی | 2024 |
| تاریخ شمسی شروع و خاتمه کنگره/همایش | 1403/02/19 الی 1403/02/20 |
| آدرس لینک مقاله/ همایش در شبکه اینترنت | https://pps5.ut.ac.ir/ |
| آدرس علمی (Affiliation) نویسنده متقاضی | Drug Applied Research Center, Tabriz University of Medical sciences, Tabriz, Iran |
| نویسنده | نفر چندم مقاله |
|---|---|
| زهرا رشیدی قلم خان | اول |
| صفر فرج نیا | دوم |
| عفت علیزاده | سوم |
| عنوان | متن |
|---|---|
| خلاصه مقاله | Osteoporosis is a common disease in the world, which is prevalent especially in aging societies. Teriparatide is a synthetic peptide amino acids 1-34 of the N-terminal of human parathyroid hormone (PTH) and approved by FDA in 2002 for treatment of osteoporosis. Ssp DnaB mini-intein with 154 amino acids is a protein which has self-splicing property. It acts as a fusion tag/fusion partner for expression of peptides and proteins in biological tools. Bioseparation technique is mediated by self-splicing inteins has become an excellent tool for affinity tag-based protein purification techniques and an alternative to conventional splicing by site-specific endoproteases. In this study the effects of Ssp DnaB mini-intein on expression and solubility of Teriparatde peptide during expression in E. coli was evaluated. The fusion of Ssp DnaB mini-intein and Teriparatide encoding sequences was achieved through the PCR technique. The fusion gene was then cloned in pET28a plasmid containing a N-terminal Histidin-tag. Subsequently the construct was introduced into E. coli BL21 strain through the process of transformation. The E. coli cells was then grown in Luria Broth (LB) medium supplemented with kanamycin antibiotic to produce the desired recombinant protein. Upon completing the solubilization process of the protein, the intein and peptide are separated under certain conditions (pH, temperature) and purified, resulting in the successful production of the desired peptide. The finding of this study revealed the potential of Ssp DnaB mini-intein in preparation of Teriparatide in E. coli which is more economical and easier way. |
| کلمات کلیدی | Teriparatide, Ssp DnaB mini-intein, Expression, E. coli |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| Expression and solubility of Teriparatide peptide in fusion of Ssp DnaB mini (1) (1).docx | 1404/01/24 | 13092 | دانلود |
| govahii.jpg | 1404/01/24 | 1264783 | دانلود |
| Poster_Template_PPS5 (0).pptx | 1404/02/07 | 3393374 | دانلود |
