چاپ صفحه |  صفحه نخست سامانه |  نویسندگان |  اطلاعات تفضیلی |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده | نفر چندم مقاله |
|---|---|
| نسیم بختیاری | اول |
| صفر فرج نیا | دوم |
| عنوان | متن |
|---|---|
| خلاصه مقاله | Introduction: Effective antimicrobial resistance can result from mutations in specific types of genes, including those encoding drug targets, drug transporters, drug transporter regulators, and antibiotic-modifying enzymes. Consequently, these acquired resistance contribute to the development of antimicrobial resistance in bacteria. Various strategies are used to overcome these problem, among them, CRISPR/cas9 based techniques are novel, accurate and rapid methods. Clustered regularly interspaced short palindromic repeats (CRISPR) gene-editing technology is the ideal tool of the future for treating diseases by permanently correcting deleterious base mutations or disrupting resistance-causing genes with great precision and efficiency. Method: We analyzed results from individual studies conducted from 2018 to 2024 using the search terms' (CRISPR) gene-editing technology ' ,' infection disease' and 'Antimicrobial resistance' in major medical databases. Result: Here, we conducted an integrated and comprehensive study of medical and biotechnological databases for application of genetic manipulation methods to overcome antimicrobial resistance. The results indicated that a variety of strategies have been developed in order to combat antibiotic-resistant bacteria, including the development of new antibiotics, bacteriophages that lyse and target these bacteria, antimicrobial peptides. Crispr/cas based methods are a newly emerged strategy that have been tried in several bacteria including pseudomonas aeruginosa, Klebsiella pnemonia and staphylococcus aurous with great success. Conclusion: In recent years, the CRISPR/Cas technique, derived from the bacterial immune system, has emerged as a promising tool for gene modification. This technology offers high efficiency, speed, and simplicity, and it is anticipated to revolutionize genome modification projects in the near future. In the CRISPR/Cas approach, the target DNA is introduced to the Cas9 enzyme through gene-specific single-guide RNAs (sgRNAs) thet resulted in the disruption and deactivation of the targeted gene. This breakthrough has opened new possibilities for combating antimicrobial resistance. Studies have shown that CRISPR/Cas technology can be employed to target and disrupt resistant genes within the bacterial DNA, potentially restoring the susceptible phenotype in resistant bacteria. |
| کلمات کلیدی | CRISPR/CAS Antimicrobial resistance gene-editing technology |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| ICB2024; nasim bakhtiyari.pptx | 1404/01/24 | 871568 | دانلود |
| New Microsoft Word Document.docx | 1404/01/30 | 25244 | دانلود |
| 1745742837017.jpg | 1404/02/07 | 2178773 | دانلود |
| 1745742837032.jpg | 1404/02/07 | 4700844 | دانلود |
