چاپ صفحه |  صفحه نخست سامانه |  نویسندگان |  اطلاعات تفضیلی |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده | نفر چندم مقاله |
|---|---|
| مرتضی عبدی قزلجه | اول |
| عباس مجدی سقین سرا | دوم |
| علی عابدالهی | سوم |
| حمید طایفی نصرآبادی | چهارم |
| صنم آذرهوش | پنجم |
| عنوان | متن |
|---|---|
| کلمات کلیدی | Alpha tocopherol-Cyclophosphamide-Antral follicle - Assisted reproductive |
| خلاصه مقاله | Abstract Background and Objective: The text discusses the challenge of preserving fertility in young women undergoing chemotherapy, particularly those treated with cyclophosphamide, a common chemotherapy drug that can lead to premature ovarian failure and infertility. It highlights assisted reproductive techniques, such as in vitro fertilization and ovarian tissue freezing, as key strategies for maintaining fertility. The study investigates the potential benefits of adding vitamin E to the culture medium of antral follicles from mice treated with cyclophosphamide, focusing on its effects on oxidative stress, hormone levels, and apoptosis-related gene expression. Material and Methods: In a study involving 6-8 week old adult female mice, the subjects were divided into two treatment group that received 150 mg/kg of cyclophosphamide and control group. On the fifth day of treatment, the mice were administered cyclophosphamide to isolate and culture antral follicles. These follicles were then cultured for four days in media with and without vitamin E. On the fourth day, researchers measured levels of estradiol, progesterone, SOD, GPX, TAC, and MDA using ELISA, and evaluated the expression of BAX and Bcl-2 genes through PCR. Findings: The research found that in the group treated only with cyclophosphamide, antioxidant indicators (SOD, GPX, TAC) significantly decreased, while MDA levels increased, compared to the group that received cyclophosphamide with vitamin E, which showed the opposite trend. Estradiol levels significantly increased in the vitamin E group compared to the control, while progesterone levels remained unchanged across all groups. Additionally, BAX gene expression was significantly higher in the cyclophosphamide group, while bcl-2 gene expression decreased in this group compared to the vitamin E group. Conclusion: The study found that adding vitamin E to the culture medium enhances steroidogenesis, antioxidant levels, and the expression of anti-apoptotic genes in the antral follicles of mice treated with cyclophosphamide. |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| govahi1.pdf | 1403/10/09 | 1851632 | دانلود |
| Screenshot (27).png | 1403/10/09 | 265192 | دانلود |
