چاپ صفحه |  صفحه نخست سامانه |  نویسندگان |  اطلاعات تفضیلی |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده | نفر چندم مقاله |
|---|---|
| حامد حاجی پور | دوم |
| فرناز توکلی | اول |
| عنوان | متن |
|---|---|
| کلمات کلیدی | Exosomes , Endometrium, Receptivity |
| خلاصه مقاله | Background: Endometrial exosomes carry bioactive agents to uterine epithelial cells and trophectoderm to promote implantation. This study investigates the probable molecular mechanisms by which exosomes improve endometrial receptivity. Materials and Methods: Exosomes were isolated from uterine fluid by Gradient ultracentrifugation and characterized by dynamic light scattering (DLS), transmission electron microscopy (TEM), and western blotting. Endometrial Ishikawa cell line were treated with isolated exosomes and implantation assay was performed to evaluate the effect of exosomes on the receptivity potential of endometrial cells. Finally, the expression of several endometrial receptivity markers was evaluated by realtime PCR. Results: DLS graph and TEM imaging showed that the isolated exosomes had a cup-shaped or spherical morphology with a mean size of 91.8 nm and zeta potential of -9.75 mV. Relatively strong immunoblotting bands for exosome-specific protein markers (CD-9 and CD-81) confirmed the isolation of exosomes. implantation assay revealed that treatment of endometrial cells by uterine exosomes enhances the receptivity potential of endometrial cells 1.5 ± 0.5 folds relative to the control group. Gene expression analysis showed that treatment of endometrial cells with uterine-derived exosomes results in upregulation and downregulation of leukemia inhibitory factor (LIF) and Mucin-16, respectively; however, the expression of Trophinin and insulin-like growth factor-binding protein 1 (IGFBP1) was not affected. Conclusion: These findings confirmed the vital role of exosomes in endometrial receptivity and showed that regulation of LIF and Mucin-16 expression is one of the probable mechanisms by which exosomes affect endometrial receptivity |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| Royan 2024 B.pdf | 1403/09/04 | 444593 | دانلود |
| royan 2024 B certificate.pdf | 1403/09/04 | 4063905 | دانلود |
