چاپ صفحه |  صفحه نخست سامانه |  نویسندگان |  اطلاعات تفضیلی |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده ثبت کننده مقاله | الهام نوروز دولت آبادی |
| مرحله جاری مقاله | تایید نهایی |
| دانشکده/مرکز مربوطه | کمیته تحقیقات دانشجویی |
| کد مقاله | 85748 |
| عنوان فارسی مقاله | Developing Retinal Cells from Peripheral Blood Mesenchymal Stem Cells: A Potential Strategy for Cell Therapy in Retinitis Pigmentosa Patients. |
| عنوان لاتین مقاله | Developing Retinal Cells from Peripheral Blood Mesenchymal Stem Cells: A Potential Strategy for Cell Therapy in Retinitis Pigmentosa Patients. |
| نوع ارائه | سخنرانی |
| عنوان کنگره / همایش | بیست و پنجمین کنگره ملی و یازدهمین کنگره بین المللی سالیانه پژوهش و فناوری دانشجویان علوم پزشکی کشور |
| نوع کنگره / همایش | بین المللی |
| کشور محل برگزاری کنگره/ همایش | Iran (Islamic Republic) |
| شهر محل برگزاری کنگره/ همایش | ارومیه |
| سال انتشار/ ارائه شمسی | 1403 |
| سال انتشار/ارائه میلادی | 2024 |
| تاریخ شمسی شروع و خاتمه کنگره/همایش | 1403/06/15 الی 1403/06/17 |
| آدرس لینک مقاله/ همایش در شبکه اینترنت | https://ibj.pasteur.ac.ir/browse.php?a_id=4393&sid=1&slc_lang=fa |
| آدرس علمی (Affiliation) نویسنده متقاضی | Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran |
| نویسنده | نفر چندم مقاله |
|---|---|
| الهام نوروز دولت آبادی | اول |
| عفت علیزاده | سوم |
| عنوان | متن |
|---|---|
| خلاصه مقاله | Retinitis pigmentosa (RP) is a group of inherited retinal diseases characterized by progressive degeneration of photoreceptor cells, leading to blindness. Cell therapy offers a promising approach to treating the progressive retinal degeneration observed in RP. Peripheral blood mesenchymal stem cells (PBMSCs) have emerged as a potential cell source for such therapies due to their accessibility and ability to differentiate into various cell types. This research aims to isolate and differentiate PBMSCs into retinal cells for regenerative medicine and cell therapy in RP patients. After identification of the patient with RP by an ophthalmologist and confirmation of the disease by molecular genetic techniques, including whole-exome sequencing (WES) and Sanger sequencing, PBMSCs were isolated from peripheral blood sample using Ficoll separation liquid and cultured in DMEM/F12 medium with 20% FBS. Passage 2 (P2) cells were analyzed by flow cytometry for characterization of PBMSCs by CD73, CD90, CD105, CD34 and CD45 markers. The cells were then differentiated into retinal cells in the presence of differentiation factors including taurine, retinoic acid, hbFGF, hEGF and L-glutamine. The identity of differentiated cells was confirmed by nestin, vimentin, CRX and rhodopsin expression using western blot. ANOVA and t-test were used to examine the western blot data, which were provided at least three trials. P values less than 0.05 were considered significant. The results of WES showed a homozygous deletion of 21 nucleotides (c.2541–2561 del) in the RPGR gene and was confirmed by Sanger sequencing, a novel pathogenic mutation associated with RP. Spindle-shaped PBMSCs appeared within 2–3 weeks after culture. P2 cells showed expression of CD90, CD73 and CD105, but expression of CD34 and CD45 was undetectable, confirming proper isolation of PBMSCs. After differentiation induction, western blot analysis showed that nestin, vimentin, CRX and rhodopsin proteins were highly expressed (P <0.05), which verified the differentiation of PBMSCs into retinal cells. Isolation and differentiation of PBMSCs into retinal cells offers a promising approach for cell therapy in patients with RP. By harnessing the regenerative potential of PBMSCs, it may be possible to replace lost or dysfunctional retinal cells and restore visual function in RP patients. However, further research is needed to overcome existing challenges and translate these findings into effective therapies for clinical use. |
| کلمات کلیدی | Retinitis pigmentosa, Peripheral blood mesenchymal stem cells, Cell therapy |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| pay.PDF | 1403/07/28 | 106185 | دانلود |
| certificate.jpg | 1403/07/28 | 547991 | دانلود |
| --v28n7p21-en.pdf | 1403/09/10 | 1098572 | دانلود |
