چاپ صفحه |  صفحه نخست سامانه |  نویسندگان |  اطلاعات تفضیلی |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده ثبت کننده مقاله | سید امیر مرادیان |
| مرحله جاری مقاله | تایید نهایی |
| دانشکده/مرکز مربوطه | کمیته تحقیقات دانشجویی |
| کد مقاله | 85488 |
| عنوان فارسی مقاله | تولید اسپرم آزمایشگاهی از بافت بیضه موش نوزاد با استفاده از پلاسمای غنی از فاکتورهای رشد |
| عنوان لاتین مقاله | In Vitro Sperm Production from Neonatal Mouse Testicular Tissue Using Plasma Rich in Growth Factors |
| نوع ارائه | سخنرانی |
| عنوان کنگره / همایش | 25th Royan International Twin Congress |
| نوع کنگره / همایش | بین المللی |
| کشور محل برگزاری کنگره/ همایش | |
| شهر محل برگزاری کنگره/ همایش | تهران |
| سال انتشار/ ارائه شمسی | 1403 |
| سال انتشار/ارائه میلادی | 2024 |
| تاریخ شمسی شروع و خاتمه کنگره/همایش | 1403/06/07 الی 1403/06/09 |
| آدرس لینک مقاله/ همایش در شبکه اینترنت | https://royancongress.com/ |
| آدرس علمی (Affiliation) نویسنده متقاضی | a Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran b Department of Reproductive Biology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran c Department of Anatomical Sciences, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran |
| نویسنده | نفر چندم مقاله |
|---|---|
| سید امیر مرادیان | اول |
| منصوره موحدین | دوم |
| عنوان | متن |
|---|---|
| کلمات کلیدی | Testicular organ culture, PRGF, In vitro spermatogenesis |
| خلاصه مقاله | Introduction: In vitro spermatogenesis using Knockout serum replacement (KSR) does indeed have some limitations, including its ineffectiveness for all strains of mice and other species. Therefore, developing a suitable media for different strains and species is of high importance. This study investigates the potential use of plasma rich in growth factors (PRGF) as a serum substitute in the media for neonatal NMRI mouse testicular tissue. Methods: Testicular tissue fragments were cultured using the gas-liquid interphase method on agarose gel for 42 days. The experimental group's media was composed of α-MEM enriched with 5% PRGF (optimal concentration), while the control group contained α-MEM with 10% KSR. The integrity of seminiferous tubules was assessed using a scoring system (1-4, worst to best). Immunofluorescence assays were performed using primary antibodies against PLZF, SYCP-3, and ACRBP to identify spermatogonial stem cells, spermatocytes, and sperm-like cells, respectively. Proliferation (Ki67), pro-apoptotic (Bax), and anti-apoptotic (Bcl-2) markers were also evaluated. Results: After 42-day culture, tissues were mechanically dissociated and flagellated sperm was observed into suspension in 3 out of the 4 samples cultured with 5% PRGF. Histological examinations indicated differentiation of germ cells up to the elongated spermatid. The percentage of tubules with good and best preserved integrity scores (3-4) was significantly higher in the 5% PRGF compared to 10% KSR. Furthermore, the 5% PRGF media promoted a higher mean number of PLZF+, SYCP3+, ACRBP+, and Ki67+ cells per tubule, indicating enhanced spermatogenesis and cellular proliferation. The mean fluorescence intensity of Bax was significantly higher in the KSR group, while Bcl-2 was higher in the 5% PRGF group, although not statistically significant. Conclusion: This study demonstrates that a media containing 5% PRGF can induce complete in vitro spermatogenesis in NMRI mice, offering a promising alternative to KSR-supplemented media. |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| New Microsoft PowerPoint Presentation.pdf | 1403/06/27 | 110097 | دانلود |
| 1.docx | 1403/07/11 | 129587 | دانلود |
