چاپ صفحه |  صفحه نخست سامانه |  نویسندگان |  اطلاعات تفضیلی |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده | نفر چندم مقاله |
|---|---|
| سید امیر مرادیان | اول |
| محمد نوری | سوم |
| زهرا امیرخانی | دوم |
| عنوان | متن |
|---|---|
| کلمات کلیدی | Testicular organ culture, ACS, In vitro spermatogenesis, Testicular tissue |
| خلاصه مقاله | Introduction: In vitro spermatogenesis requires a specialized media to support the complex process of sperm development and maturation. Introduced culture media, such as knockout serum replacement (KSR), have several restrictions such as low efficiency compared to in vivo conditions and ineffectiveness for all mice strains and other species. The aim of this study is to evaluate the effects of autologous conditioned serum (ACS) in supporting in vitro mouse spermatogenesis. Methods: Immature testicular tissue of NMRI mice were cultured on agarose gel in two media including α-MEM enriched with 5% ACS and 10%ACS for 42 days. The spermatogenic tubules integrity was assessed applying a scoring system (1-4, worst to best). Immunofluorescence tests were carried out with primary antibodies targeting PLZF, SYCP-3, ACRBP, SOX9, and STAR to distinguish between spermatogonial stem cells, spermatocytes, sperm-like cells, Sertoli cells, and Leydig cells, respectively. Additionally, Ki67 and caspase 3 markers were analyzed to examine proliferating and apoptotic cells, respectively. Results: After culturing tissues for 42 days and mechanically dissociating them, round spermatid-like cells were observed in fragments cultured in 5%ACS. Morphological evaluation revealed that spermatogonial stem cells differentiated up to the round spermatid in 5%ACS. The 5% ACS media also preserved significantly higher percentage of tubules based on the integrity scoring (3-4) compared to the 10% ACS. Moreover, quantitative analysis showed a significant increase in the number of PLZF+, SYCP3+, ACRBP+, SOX9+, and Ki67+ cells per tubules in 5% ACS compared to 10% ACS. The average number caspase3+ per tubule was significantly higher in the 10% ACS than in the 5% ACS. However, there was no significant difference in the number of STAR+ cells per tubule between the two media. Conclusion: This study showed that media supplemented with 5% ACS compared to 10% ACS can more effectively induce in vitro spermatogenesis up to round spermatid stage in NMRI mice. |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| 1.docx | 1403/07/09 | 112431 | دانلود |
| 1.pdf | 1403/07/11 | 236208 | دانلود |
