چاپ صفحه |  صفحه نخست سامانه |  نویسندگان |  اطلاعات تفضیلی |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده | نفر چندم مقاله |
|---|---|
| پریسا حسن پور | اول |
| سولماز ثاقب اصل | دوم |
| علی مطاع | سوم |
| محمد رحمتی | چهارم |
| رضا رهبرقاضی | پنجم |
| حسین احمدی تفتی | هفتم |
| شهرام ربانی | هشتم |
| عنوان | متن |
|---|---|
| خلاصه مقاله | Background Here, the angiogenesis properties of mesenchymal stem cell (MSC) mitochondria were investigated in a rat model of myocardial infarction. Material and Methods Mesenchymal stem cells were pre-treated with mitochondria-induction compounds metformin (Met; 50 μM), and dichloroacetic acid (DCA; 40 μM). After the isolation of mitochondria, the isolated particles were characterized and incorporated (~2 × 107 particles) inside the alginate (3%)-gelatin (1%) hydrogel containing 1 μM pyrrole in the presence of FeCl3. The physicochemical properties of the final composite were monitored using several assays such as FTIR, swelling, biodegradation, porosity tests, and SEM. Mitochondria-loaded hydrogels (~2 × 107 particles/100μl) were injected into the border zone of infarcted myocardium induced by LAD ligation. After two weeks, rats were euthanized and mitochondrial uptake and angiogenesis status were monitored using histological examination. ResultsResults Data showed appropriate physicochemical (swelling rate, biodegradation, porosity) and cytocompatibility properties of alginate-gelatin pyrrole hydrogel evaluated by SEM, FTIR, and MTT assays. Flow cytometry analysis indicated high-rate purity and functionality of isolated mitochondria. Masson’s Trichrome staining and ECG analysis indicated appropriate myocardial infarction in the control rats. Immunohistochemistry analysis showed the stimulation of angiogenesis in the infarcted area (vWF+ capillaries↑, and α-SMA+ arterioles↑) in rats that received mitochondria-loaded hydrogel compared to mitochondria and hydrogel groups (p<0.05). Data showed a significant increase in anterior wall thickness in the mitochondria-loaded hydrogel group (p<0.05). Conclusion Conclusion Mitochondria-loaded hydrogels are suitable delivery platforms for the induction of angiogenesis and reduction of fibrosis in ischemic myocardium. |
| کلمات کلیدی | Mesenchymal Stem Cells; Mitochondria; Electroconductive Alginate-Gelatin/Pyrrole Hydrogel; Rat Myocardial Infarction; Angiogenesis |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| CamScanner 08-31-2024 06.36n_1.jpg | 1403/06/10 | 780338 | دانلود |
| Final Scientific Program- 20th Congress on Stem Cell Biology and Technology (1).pdf | 1403/06/10 | 323911 | دانلود |
| Abstract (1).pdf | 1403/06/10 | 88487 | دانلود |
