چاپ صفحه |  صفحه نخست سامانه |  نویسندگان |  اطلاعات تفضیلی |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده ثبت کننده مقاله | محمد رضا اردلان |
| مرحله جاری مقاله | تایید نهایی |
| دانشکده/مرکز مربوطه | مرکز تحقیقات کلیه |
| کد مقاله | 85045 |
| عنوان فارسی مقاله | miR-186, a potential noninvasive diagnostic biomarker of Membranous nephropathy in urinary |
| عنوان لاتین مقاله | miR-186, a potential noninvasive diagnostic biomarker of Membranous nephropathy in urinary |
| نوع ارائه | سخنرانی |
| عنوان کنگره / همایش | 61st ERA Congress |
| نوع کنگره / همایش | بین المللی |
| کشور محل برگزاری کنگره/ همایش | Sweden |
| شهر محل برگزاری کنگره/ همایش | Stockholm |
| سال انتشار/ ارائه شمسی | 1403 |
| سال انتشار/ارائه میلادی | 2024 |
| تاریخ شمسی شروع و خاتمه کنگره/همایش | 1403/03/03 الی 1403/03/06 |
| آدرس لینک مقاله/ همایش در شبکه اینترنت | https://www.era-online.org/events/stockholm-2024/ |
| آدرس علمی (Affiliation) نویسنده متقاضی | Kidney Research Center, Tabriz University of Medical Sciences, Tabriz, Iran |
| نویسنده | نفر چندم مقاله |
|---|---|
| نگین فرزامی کیا | اول |
| محمد رضا اردلان | دوم |
| سپیده زنونی واحد | سوم |
| عنوان | متن |
|---|---|
| خلاصه مقاله | Membranous nephropathy (MN) is one of the most common causes of nephrotic syndrome, and is associated with podocyte damage and proteinuria. Invasive biopsy is the gold-standard diagnostic method for this disease. The use of specific biomarkers, such as microparticles (MPs) originating from damaged podocytes released in a patient's urine, has been proposed as an early, noninvasive, and repetitive diagnostic method. According to the previous studies, MPs derived from podocytes are present in the urine of patients with different glomerulonephritis, such as diabetic nephropathy, lupus nephritis, renal injury in preeclampsia, and renovascular hypertensive disorders. The analysis of urinary microparticle-derived miRNAs from patients with kidney diseases can help detect diagnostic biomarkers. This study aimed to analyze podocyte damage-related miRNAs in MN, miR-30c, miR-135, and miR-18 in the urinary MP of MN patients to identify appropriate candidate biomarkers for MN Method Urine samples from 30 patients with membranous nephropathy and 16 healthy individuals were collected to extract podocyte MPs using ultracentrifugation. Characterization of these particles was determined using the specific protein marker of MPs, TSG-101, and the podocyte-specific marker Nephrin, by western blotting, and SEM was used to check the size and morphology. The expression levels of microRNAs extracted from podocyte MPs, miR-30c, miR-135, and miR-186, were also evaluated by real-time PCR. Results The evaluation of the characteristics of MPs showed that there were many particles with a size of more than 100 nm in the urine sample, and there were higher levels of the specific markers TSG-101 and Nephrin in the urine samples of MGN patients than in the control samples (P < 0.001). The expression level of miR-186 was compared between the three studied groups and based on the results of the expression of this miRNA in the group of membranous nephropathy patients compared to healthy people group, there was a significant increase in expression (P = 0.000) Conclusion This study showed that although the expression levels of miR-186 in podocyte MPs samples extracted from the urine of patients with MN were significantly changed compared to the studied controls, more studies should be performed to determine the diagnostic values. |
| کلمات کلیدی |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| AbstractAuthorCertificate_MArdalan_2.pdf | 1403/04/29 | 189972 | دانلود |
| miR-186.pdf | 1403/04/29 | 288166 | دانلود |
| CMECertificate_MArdalan_4.pdf | 1403/04/29 | 403278 | دانلود |
