چاپ صفحه |  صفحه نخست سامانه |  نویسندگان |  اطلاعات تفضیلی |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده | نفر چندم مقاله |
|---|---|
| فاطمه تمجید | اول |
| ژاله برار | دوم |
| یلدا رهبرسعادت | سوم |
| علیرضا استاد رحیمی | چهارم |
| عنوان | متن |
|---|---|
| خلاصه مقاله | Background and Aim: Pancreatic cancer is known as the most malignant tumor. Surgery and chemotherapy are potentially curative treatments for pancreatic cancer patients with a low response rate. Recently, a growing interest has been dedicated to natural products. Sweet apricot kernel exerts a number of pharmacological activities; however, the anti-cancer effect, related mechanisms and its active compounds were rarely investigated. In this research, we aimed to evaluate the cytotoxic/apoptotic effects of sweet apricot kernel ethanolic extract (SAEE). Methods: The extract was prepared by maceration and its chemical composition analyzed by gas chromatography-mass spectrometry (GC-MS). The biological effects of SAEE on cancerous PANC-1 and 293/KDR normal cells were investigated by MTT assay, DAPI and AnnexinV/PI staining. The expression of pro- and anti-apoptotic genes was evaluated by Real-time q-PCR analysis. Results: The SAEE showed selective growth inhibitory activity against PANC-1 cells in a dose- and time-dependent manner. DAPI staining and flow cytometry revealed nucleus fragmentation and elevation of apoptotic cells, respectively. Also, a significant decrease of Bcl-2/Bax ratio and up-regulation of caspase-3 expression indicated the induction of apoptosis in PANC-1 cells, but not in 293/KDR cells. These results suggest that SAEE could induce apoptosis in cancer cells via a mitochondrial dependent pathway. Furthermore, GC-MS analysis showed that the SAEE is rich in gamma-sitosterol and gamma-tocopherol. Conclusion: Our findings suggest that due to the selective impacts of SAEE on PANC1 cells, it could be considered as a promising adjuvant for pancreatic cancer therapy. Though, the potent anti-cancer effects of main components of SAEE should be further investigated. |
| کلمات کلیدی | Keywords: Pancreatic cancer, Sweet apricot kernel, Apoptosis, γ-sitosterol, γ-tocopherol |
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