چاپ صفحه |  صفحه نخست سامانه |  نویسندگان |  اطلاعات تفضیلی |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده | نفر چندم مقاله |
|---|---|
| آلکا حسنی | اول |
| معصومه مدحی لاله | دوم |
| القار سلطانی | سوم |
| محمد یوسف معمار | چهارم |
| اکبر حسنی | پنجم |
| عنوان | متن |
|---|---|
| کلمات کلیدی | Pseudomonas aeruginosa, Efflux pump, mexB; mexY, Chitosan nanoparticles |
| خلاصه مقاله | Background and Aim: Though nanoparticles have found their place in the modern scientific therapeutic world however, before they step into treatment strategies it is necessary to determine their effects either alone or with antibiotics in lowering antibiotic resistance. This study aimed at to evaluate the antibacterial activity of chitosan nanoparticles (NPs) alone and in combination with ciprofloxacin against P.aeruginosa and its efficacy of to alter the expression level of efflux pumps. Method: The antibacterial activities of chitosan, chitosan NPs and its combination to ciprofloxacin (CNC) were evaluated through the use of micro dilution broth, disc diffusion and drop diffusion methods. In addition, the toxicity of the synthesized materials was calculated by MTT assay. To conduct the experiments of the expression of target genes of mexB, and mexY, initially antibiotic resistant P. aeruginosa (PAO1) strains were exposed to the chitosan in its sub inhibitory levels, then RNA was extracted to study the antibacterial effects of the nanoparticles in relation to the expression of the efflux pump using real-time PCR. Results: The result showed because of the difficulty of movement of the synthetic substances on the Agar medium, antibacterial activities of chitosan NPs, CNC, against P.aeruginosa were not the same using three analytic methods. Micro dilution broth was the most amenable technique among the three procedures. Chitosan and its nanoparticle forms combined with antibiotics, did not show toxic properties on stem cells by MTT assay. The result also showed efflux pump expression of mexB and mexY in P. aeruginosa decreased after treating them with chitosan, chitosan nanoparticle, so they are good candidate as an efflux pump inhibitor. Conclusion: The outcomes showed the higher antibacterial and anti-efflux pump activity of chitosan against P. aeruginosa. Minimum inhibitory concentration (MIC) levels of ciprofloxacin was found to have increased after exposure to the nanoparticle. |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| 25_0_Alka Hasani.jpg | 1402/11/23 | 356585 | دانلود |
| 22_0_Alka Hasani.jpg | 1402/11/23 | 322890 | دانلود |
| chitosan and P.aeruginosa.doc | 1402/11/23 | 29184 | دانلود |
