چاپ صفحه |  صفحه نخست سامانه |  نویسندگان |  اطلاعات تفضیلی |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده | نفر چندم مقاله |
|---|---|
| مصطفی اکبرزاده خیاوی | اول |
| اعظم صفری | دوم |
| عنوان | متن |
|---|---|
| کلمات کلیدی | Superparamagnetic Nanoparticles, Near-Infrared, Panitumumab , Colorectal Cancer |
| خلاصه مقاله | Introduction : This study presents an innovative and efficient tumor-targeted nanotherapeutic using superparamagnetic nanoparticles (SPIONs) conjugated with anti-EGFR Panitumumab. We conducted comprehensive characterizations and assessments of this nanotherapeutic to evaluate its biological effects and photothermal properties in SW-480 colorectal cancer cells. To assess the efficacy of this nanobiosystem in combination with photothermal therapy (PTT), we employed low-intensity near-infrared (NIR) laser irradiation. Methods: Targeted SPION-Pan nanomedicine was synthesized and characterized by DLS, FTIR, UV-Vis, and TEM. Its biological impacts in the SW-480 colon cancer cells were evaluated with MTT assay and DAPI and apoptosis assay by flow cytometry on KRAS mutant SW-480 colorectal cancer cells combined with photothermal therapy (PTT).To determine the efficacy of targeted nanomedicine combined with the PTT, low doses of the near-infrared (NIR) laser irradiation were utilized at different power densities of 16–64 joule/second/square meter J/(s m2). Result: The study findings demonstrated that the targeted nanobiosystem resulted in diminished cell viability and the induction of apoptosis in the SW480 cell line. Furthermore, our investigation showed that the combination therapy (PTT + SPION-Pan) had a synergistic effect on promoting cancer cell apoptosis. Discussion & Conclusion: We introduced a potent nanoformulation of panitumumab in combination therapy with PTT, demonstrating a remarkable capacity to overcome resistance to anti-EGFR monoclonal antibodies and induce apoptosis in colorectal cancer cells, outperforming the efficacy of free antibodies. Altogether, the designed nanobiosystem might serve as an effective treatment strategy to suppress or minimize the resistance of KRAS mutant CRC cells and sensitize them to immunotherapy and chemotherapy. |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| Dr Akbarzadeh.pdf | 1402/10/11 | 522566 | دانلود |
| certificate (5).pdf | 1402/10/11 | 132349 | دانلود |
