چاپ صفحه |  صفحه نخست سامانه |  نویسندگان |  اطلاعات تفضیلی |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده | نفر چندم مقاله |
|---|---|
| لیلا رهبرنیا | سوم |
| محمدحسین غفاری اقدم | اول |
| عنوان | متن |
|---|---|
| کلمات کلیدی | Staphylococcus aureus, vancomycin-resistant, melittin peptide, synergism |
| خلاصه مقاله | Multidrug resistance Staphylococcus aureus(MDR) strains are increasingly emerging as serious threat for public health. Moreover, the use of vancomycin as last resort antibiotic for the treatment of MDR infections is limited because of severe toxicity in high doses The antimicrobial activity of melittin is now confirmed against a wide variety of bacteria. However, the high toxicity is one of the limiting factors for the therapeutic application of Melittin. The combinational therapy is one of strategies used to reduce toxicity of drugs because decrease of dose of drug. In this study, the antibacterial effect of melittin peptide in combination with vancomycin was evaluated to control vancomycin resistant(VRSA) clinical isolates. Methods: In this study, melittin was purified from Iranian honey bee venom by reversed-phase HPLC. Minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) of antimicrobial agents were determined against VRSA isolates. The cytotoxicity of melittin on HUVEK cells and human red blood cells (RBCs) was evaluated at their synergistic concentrations. Results: MIC and MBC values for melittin were equal to 16 and 32, respectively. Results also showed that the interaction of melittin with vancomycin was highly synergistic. Induced synergism led to a decrease in melittin and vancomycin concentrations by 32 and 16-folds, respectively. So that, only 0.25 ?g/mL melittin in combination with 0.125 ?g/mL vancomycin was sufficient to inhibit VRSA isolates growth. Based on Hemolytic and MTT assay results, Melittin did not show toxic effects at 0.25?g/ml as the synergistic concentration. Conclusion: our findings indicated that melittin showed highly synergistic effects with vancomycin with reduced toxicity. Therefore, the combination of melittin and traditional antibiotics could be a promising strategy for the treatment of VRSA infections. |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| abstract synegism.pdf | 1402/10/11 | 370425 | دانلود |
| Ism_certificate (2).pdf | 1402/10/11 | 22430201 | دانلود |
