Anopheles stephensi Host Feeding Detection by Multiplex PCR

Anopheles stephensi Host Feeding Detection by Multiplex PCR


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نویسندگان: مدینه عباسی

عنوان کنگره / همایش: International Congress on Vector-Borne Diseases and National Congress on Medical Entomology , Iran (Islamic Republic) , Tehran , 2023

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نویسنده ثبت کننده مقاله مدینه عباسی
مرحله جاری مقاله تایید نهایی
دانشکده/مرکز مربوطه بیماری های عفونی و گرمسیری
کد مقاله 83641
عنوان فارسی مقاله Anopheles stephensi Host Feeding Detection by Multiplex PCR
عنوان لاتین مقاله Anopheles stephensi Host Feeding Detection by Multiplex PCR
نوع ارائه پوستر
عنوان کنگره / همایش International Congress on Vector-Borne Diseases and National Congress on Medical Entomology
نوع کنگره / همایش بین المللی
کشور محل برگزاری کنگره/ همایش Iran (Islamic Republic)
شهر محل برگزاری کنگره/ همایش Tehran
سال انتشار/ ارائه شمسی 1402
سال انتشار/ارائه میلادی 2023
تاریخ شمسی شروع و خاتمه کنگره/همایش 1402/08/30 الی 1402/09/02
آدرس لینک مقاله/ همایش در شبکه اینترنت https://icvbd2023.ir/fa/
آدرس علمی (Affiliation) نویسنده متقاضی nfectious and Tropical Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

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مدینه عباسیاول

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عنوان متن
خلاصه مقالهIntroduction: Anopheles stephensi is an important mosquito species in malaria epidemiology due to its high level of anthropophily, or preference for feeding on humans. This increases the risk of disease transmission and makes it a significant vector of malaria in many regions. Detecting the blood meal host of An. stephensi is important for understanding the transmission dynamics of malaria and designing effective control programs. This information can help identify which host species are contributing most to disease transmission and target control measures accordingly. Additionally, identifying non-human blood meal hosts can provide insight into the mosquito's ecology and behavior, which can inform strategies for reducing its population and limiting its impact on human health. Method: The Collins method was used for DNA extraction from the samples collected from Bandar Abbas, followed by the Multiplex-PCR method using specific primers for humans and potential animal hosts in the region. The resulting products were separated by electrophoresis based on differences in band sizes of potential hosts. Results and discussion: In total, 77 samples of An. stephensi were used to determine the source of blood feeding. Among potential human hosts, the highest proportion (29.9%) was observed. The lowest proportion of blood feeding was attributed to birds (3.11%) followed by dogs (6.2%). In our study, among the hosts that An. stephensi fed on for their blood needs, humans had the highest proportion. Determining the source of blood feeding in malaria epidemiology calculations and designing control programs is of particular importance. The high tendency of mosquitoes to feed on humans increases the capacity for disease transmission
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