Determination of the feeding behavior of Phlebotomus sergenti using multiplex PCR and tent-baited traps in a new focus of Anthroponotic Cutaneous Leishmaniasis in the southeast of Iran

Determination of the feeding behavior of Phlebotomus sergenti using multiplex PCR and tent-baited traps in a new focus of Anthroponotic Cutaneous Leishmaniasis in the southeast of Iran


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پژوهان
صفحه نخست سامانه
نویسندگان
نویسندگان
اطلاعات تفضیلی
اطلاعات تفضیلی
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نویسندگان: مدینه عباسی

عنوان کنگره / همایش: International Congress on Vector-Borne Diseases and National Congress on Medical Entomology , Iran (Islamic Republic) , Tehran , 2023

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نویسنده ثبت کننده مقاله مدینه عباسی
مرحله جاری مقاله تایید نهایی
دانشکده/مرکز مربوطه بیماری های عفونی و گرمسیری
کد مقاله 83639
عنوان فارسی مقاله Determination of the feeding behavior of Phlebotomus sergenti using multiplex PCR and tent-baited traps in a new focus of Anthroponotic Cutaneous Leishmaniasis in the southeast of Iran
عنوان لاتین مقاله Determination of the feeding behavior of Phlebotomus sergenti using multiplex PCR and tent-baited traps in a new focus of Anthroponotic Cutaneous Leishmaniasis in the southeast of Iran
نوع ارائه پوستر
عنوان کنگره / همایش International Congress on Vector-Borne Diseases and National Congress on Medical Entomology
نوع کنگره / همایش بین المللی
کشور محل برگزاری کنگره/ همایش Iran (Islamic Republic)
شهر محل برگزاری کنگره/ همایش Tehran
سال انتشار/ ارائه شمسی 1402
سال انتشار/ارائه میلادی 2023
تاریخ شمسی شروع و خاتمه کنگره/همایش 1402/08/30 الی 1402/09/02
آدرس لینک مقاله/ همایش در شبکه اینترنت https://icvbd2023.ir/fa/
آدرس علمی (Affiliation) نویسنده متقاضی Infectious and Tropical Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Ira

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مدینه عباسیچهارم

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عنوان متن
خلاصه مقالهBackground Phlebotomine sand flies are blood-feeding insects transmitting various Leishmania species (Family: Trypanosomatidae) to humans and animals. The blood preference of sand flies is crucial to identify potential reservoir hosts of Leishmania parasites.This study aimed to determine feeding behaviors of Phlebotomus sergenti Parrot, in a new focus of Anthroponotic Cutaneous Leishmaniasis in Bam County, southeast Iran. Material and methods Reference Results Conclusion Two methods were used to determine the feeding behavior of Ph. sergenti. In the first method, blood-fed sand flies were captured using a mouth aspirator in human and animal dwellings and consequently, blood meal identification was made using Multiplex PCR. The results were used for calculating Host Feeding Index (HFI) and Forage Ratio (FR) parameters. In the second method, human ( Homo sapiens), goat ( Capra aegagrus), cattle ( Bos taurus), chicken ( Gallus gallus) and dog ( Canis lupus) were used as baits in tent-baited traps to determine the feeding behavior of Ph. sergenti. Multiplex PCR analysis revealed that the most frequent blood in the stomach of sand flies’ were from chicken, but the calculation of the FR revealed that this species prefers canine and poultary blood as meal. Human and animal tent- baited traps revealed that most Ph. sergenti were attracted to chicken rather than the other hosts. Sand flies are attracted to animals for various reasons such as eating blood, mating on their bodies and laying eggs on their feces. Molecular methods are effective and accurate methods to determine the type of host that sand fly fed on, but they do not show host preferences. The results of the molecular analysis, along with the calculation of HFI and FR, can determine the preferred host of sand flies. The current study revealed that dogs, the secondary reservoir of ACL in Iran, is the first preferred host of Phlebotomus sergent
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