چاپ صفحه |  صفحه نخست سامانه |  نویسندگان |  اطلاعات تفضیلی |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده | نفر چندم مقاله |
|---|---|
| عفت علیزاده | دوم |
| بهزاد فلاحی مطلق | سوم |
| نصرت اله ضرغامی | چهارم |
| مریم قاسمی | اول |
| عنوان | متن |
|---|---|
| کلمات کلیدی | Adipose tissue-derived mesenchymal stem cells (AT-MSCs), cell cycle, ciliary neurotrophic factor (CNTF), differentiation |
| خلاصه مقاله | Introduction Mesenchymal stem cells (MSCs) as primary cells contribute to the treatment of some diseases as well as neural diseases in particular for retinal cell therapy. The aim of this study was to evaluate the effects of ciliary neurotrophic factor supplementation on Adipose tissue-derived mesenchymal stem cells (AT-MSCs) toward retinal lineage. Material & Methods The AT-MSCs were treated with different concentrations of CNTF (5, 25, 100 ng/µl), followed by MTT, BrdU assay, FACS cell cycle analysis, and Real-time PCR of differentiation markers (neuron-specific enolase (NSE) and neuronal nuclei (NeuN)). Results Our findings showed that CNTF stopped the proliferation of AT-MSCs in doses (50 and 100 ng/mL). An arrest in AT-MSCs in the G0/G1 phase of the cell cycle was detected by CNTF treatment. stemness genes transcription was reduced in AT-MSCs (P < .05) and levels of NSE and NeuN increased (P < .05) indicating a commitment to retinal lineage. Conclusion The findings of our study propose promising potential effects of CNTF in differentiation-inducing function for AT-MSCs as primary cells. These treated cells could be a candidate for cell therapy in retinal disease in the future. |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| certificate.pdf | 1403/08/19 | 149677 | دانلود |
| poster bartar.pdf | 1403/08/19 | 575394 | دانلود |
| ABSTRACT.MG.docx | 1403/08/20 | 14422 | دانلود |
| abstract.pdf | 1403/08/20 | 686062 | دانلود |
