چاپ صفحه |  صفحه نخست سامانه |  نویسندگان |  اطلاعات تفضیلی |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده | نفر چندم مقاله |
|---|---|
| فرشته راهدان | اول |
| زینب چهارلشکر | دوم |
| عفت علیزاده | پنجم |
| فاطمه عابدی | سوم |
| عنوان | متن |
|---|---|
| کلمات کلیدی | Key words: Cellulose nanocrystals, modified CNCs , siRNA delivery , cancer therapy |
| خلاصه مقاله | Introduction Recently, gene therapy based on interfering RNAs (siRNAs) has been used as a potential new drug for cancer treatment. However, according to report of the clinical trials, siRNA delivery has several limitations, including ineffective intracellular delivery, instability in blood flow, and off-target effect and unwanted tissue accumulation and high toxicity. High efficiency delivery vectors are required for targeted safe siRNA delivery. Cellulose nanocrystals as a natural biopolymer with high stability, biocompatibility, bio renewability and potential for functional changes at the nanoscale can be effectively used for siRNA delivery. Cellulose nanocrystals has a negative charge and surface-modified cellulose nanocrystals (CNCs) which inserted positively charged groups were developed for efficient delivery of RNAi into cancer cells. This review discusses the recent progress future prospects, and challenges in the delivery of siRNAs mediated by CNC and its derivatives in cancer therapy. Method Original articles published since 2007 on cellulose-based nanocarriers in the field of RNA carrier for cancer treatment were searched from Google Scholar, Web of Science, Scopus, Elsevier, and PubMed databases. Using these data, the application, properties, and challenges of cellulose-based nanocarriers were discussed. Results & Discussion Cellulose is a natural biopolymer, a great part of our food, and can be tolerated in human body without any undesired toxicity. However, at the moment of complexation with siRNA, the major issue is its negative charge which can cause repulsion of siRNA. New nanocomplexes are synthesized with surface charge changes in order to load negatively charged. RNAi while maintaining the inherent properties of cellulose. Cellulose-based nanocomplexes have physicochemical properties selected for effective controlled release of RNAi into cells and have enzymatic stability against degradation. Recently, the delivery platform based on cellulose nanocrystals was synthesized using chemical modification and sequential hydrothermal desulfation process. Subsequently, designed siRNA formed complexes with Mg2+ chelation and loaded with modified CNCs by electrostatic interaction. In this regard, high loading and release of siRNA in optimal cytoplasmic environment was reported. The results suggest CNCs as a suitable carrier could be used in terms of optimal delivery of therapeutic RNAi to cancer cells. |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| 25_1_Fereshteh Rahdan1, Zeinab Chaharlashkar1, Fatemeh Abedi2, Dariush Rahdan3, Effat Alizadeh1_.jpg | 1402/10/04 | 365545 | دانلود |
| FR-EA.jpg | 1402/10/04 | 881401 | دانلود |
