چاپ صفحه |  صفحه نخست سامانه |  نویسندگان |  اطلاعات تفضیلی |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده | نفر چندم مقاله |
|---|---|
| رضا قوطاسلو | اول |
| سودا ذوقی | دوم |
| حامد ابراهیم زاده لیل آبادلو | سوم |
| عنوان | متن |
|---|---|
| کلمات کلیدی | E. coli, antibiotic resistant genes, PCR, Azerbaijan, Iran |
| خلاصه مقاله | BACKGROUND AND OBJECTIVES Antibiotic resistance increases the prolonged consequences and mortality caused by E. coli infections. The emergence and expression of resistant genes is a potential factor for the stability of this pathogen against antibiotics. Hence, the present study was designed to investigate the prevalence of antibiotic resistance genes in E. coli isolates from Tabriz, Iran. MATERIALS AND METHODS In this study, 141 females and 78 males aged 1 to 89 from surgery, internal, intensive care unit, and pediatric wards of Tabriz hospitals were enrolled. E. coli was isolated from 219 biological samples (urine, blood, wounds, peritoneum, and respiratory tracts) by culture on sheep blood agar or MacConkey agar and microbial detection standards. The genomic DNA was extracted using cetyltrimethylammonium bromide and resistance genes were identified by polymerase chain reaction method. RESULTS AND DISCUSSION In the molecular investigation, at least 4 high risk genes inducing resistant for E. coli isolates were reported in each antibiotic group. The interpretation of data for each of the antibiotic groups indicates that blaCTXM-15 (70%) among positive beta-lactamases, tetB (31.6%) in tetracycline, fosC (40%) in fosfomycin, OqxB (34%) in fluoroquinolone, ArmA (12.96%) in aminoglycosides, and Sul1 (69.5%) in co-trimoxazole combination are genetic indexes of antibiotic resistance. CONCLUSION The genes encoding the destructive factors of antibiotics had a significant prevalence in E. coli isolates. The blaCTXM-15 inducing beta-lactam resistance was the most common gene among our clinical isolates. Due to the possibility of development of resistance genes with chromosomal or plasmid origin, the consumption of antibiotics based on approved global standards has a high priority. |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| E.coli 2 (Booklet).pdf | 1402/08/20 | 406664 | دانلود |
| E.coli 2 (certificate).pdf | 1402/08/20 | 1446230 | دانلود |
