چاپ صفحه |  صفحه نخست سامانه |  نویسندگان |  اطلاعات تفضیلی |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده | نفر چندم مقاله |
|---|---|
| ناهیده نزدیک بین یامچی | اول |
| رضا رهبرقاضی | دوم |
| مهدی مهدی پور | سوم |
| عنوان | متن |
|---|---|
| کلمات کلیدی | Premature Ovarian Insufficiency; Bone Marrow; Endothelial Cells; Mesenchymal Stem Cells; Fibrosis; Regeneration |
| خلاصه مقاله | Background: Until today many attempts were done to restore the ovarian function in POI patients with varied cell types, however, the regeneration potential of endothelial cells versus mesenchymal of stem cells has not been fully investigated. Objective: This study aimed to explore the potential therapeutic effects of marrow derived CD144+ endothelial cells (ECs) and CD146+ mesenchymal stem cells (MSCs) in experimentally produced POI rats. Materials and Methods: POI-like conditions were produced in rats using intraperitoneal injection of cyclophosphamide, 200 mg/kg in the first day and 8 mg/kg/day for the 2nd to 14th day. After POI induction, rats were assigned into Control POI, POI + CD146+ MSCs and POI + CD144+ ECs groups. Rats were subsequently administrated with about 15 x 104 cells/10μL/ovary in the relevant groups. After 4 weeks, ovarian tissues were subjected to histopathological examination and serum samples were used for FSH, LH, and E2 hormone levels analysis. The fibrotic status of the tissues were also investigated via Masson’s trichrome staining. Results: Our findings revealed that administration of CD146+ MSCs and CD144+ ECs in POI rats elevated the number of morphologically normal follicles and reduced atretic follicles (p<0.01). In addition to these improvements, the presence of collagen fibers has lessened after intervention. Likewise, cell transplantation in POI rats suppressed the increased FSH and LH levels (p<0.05), however, E2 levels was raised in CD146+ MSCs and CD144+ ECs recipient compared to control POI rats, illustrating the restoration of ovarian function. Conclusions: The transplantation of marrow derived CD146+ and CD144+ cells restored ovarian tissue function in POI rats with different regenerative outcomes. |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| Certificate Yazd 1402.pdf | 1402/08/15 | 214888 | دانلود |
| davatname.pdf | 1402/08/15 | 118806 | دانلود |
| 10th_yazd_international_congress_and_student_award_on_reproductive_medicine_compressed-1 (1).pdf | 1402/08/15 | 116850 | دانلود |
