چاپ صفحه |  صفحه نخست سامانه |  نویسندگان |  اطلاعات تفضیلی |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده | نفر چندم مقاله |
|---|---|
| آرمیتا قوطاسلو | اول |
| آرزو عزیزسلطانی | دوم |
| عفت علیزاده | سوم |
| عنوان | متن |
|---|---|
| خلاصه مقاله | Introduction Hepatocellular carcinoma (HCC), a deadly form of liver cancer, is influenced by miRNAs and imbalances in redox homeostasis. Cancer cells adapt themselves to high oxidative stress by increasing self-antioxidant levels through the Nrf2/Keap1 pathway, but this can promote tumor growth and drug resistance[1]. Human embryonic kidney cells (HEK-293) are considered a suitable source of exosomes due to their transfection ease and lack of cancer-related antigens[2, 3]. This study aims to investigate whether delivering miR-365a-3p through exosomes can deactivate the antioxidant mechanism in HCC cells, potentially by increasing ROS levels[4]. Material and methods After isolation of exosomes from HEK-293, they were loaded with miR-365a-3p mimic via modified calcium chloride method. In order to evaluate the efficiency of the miR-365a-3p encapsulation, quantitative real-time PCR was used. Next, the effect of the miR-365a-3p loaded HEK-293 Exosomes on intracellular ROS, Nrf2, HO-1, and SOD1 protein, and Bcl2 mRNA levels was investigated. Results & Discussion In vitro findings demonstrated that, ExomiR-365a-3p efficiently transfected in to cytoplasm of HepG2 cells and significantly reduced the Nrf2, HO-1, and SOD1 protein level with inverse augmentation of ROS levels. A significant population of exomiR-365a-3p treated cells showed the intense decrease of Bcl2 as antiapoptotic factor. Taken together, Exosome-mediated delivery of miR-365a-3p might induce apoptosis through decreasing Nrf2 pathway proteins level and following increased oxidative stress in HepG2 cells. |
| کلمات کلیدی | MiR-365a-3p-mimic, ROS, Exosome, HepG2, Nrf2 |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| -5920127142522372269_121.jpg | 1402/08/12 | 232015 | دانلود |
| biotech13-00090447_1_230821204732.docx.pdf | 1402/08/13 | 108864 | دانلود |
| AG-EA.jpg | 1402/10/03 | 320641 | دانلود |
