چاپ صفحه |  صفحه نخست سامانه |  نویسندگان |  اطلاعات تفضیلی |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده | نفر چندم مقاله |
|---|---|
| سویل واقفی مقدم | اول |
| فاطمه عابدی | دوم |
| عفت علیزاده | سوم |
| عنوان | متن |
|---|---|
| کلمات کلیدی | Cell Sheet, Ascorbic acid, Selenium, Trolox |
| خلاصه مقاله | Objective: Enrichment of culture medium by antioxidants could help to maintain mesenchymal stem cells in high quality for subsequent applications. Also, recently developed cell sheet technology takes advantage of using antioxidant supplements to enhance extracellular matrix secretion by cells. The present study aims to optimize the fabricated cell sheet from bone marrow-derived mesenchymal stem cells (rBMSCs) under the influence of selenium and compare it whit the ones fabricated under the influence of Trolox or Ascorbic acid. Also, considering the potential of temperature-responsive PCLPEG-PCL (PCEC) with a certain molecular weight as an intelligent surface for controlling cell sheet attachment and detachment we use it as a substrate for cell sheet engineering. Method: The substrate was prepared by the ring-opening copolymerization method and characterized in terms of physicochemical properties. Each well of the culturing plate was coated with the synthesized substrate. In the following step, the rBMSCs were cultured on the surface of the coated polymer and let be expanded in five in vitro microenvironments containing selenium, Trolox, ascorbic acid, selenium along with ascorbic acid, and routine medium. After the harvesting of resulted cell sheets by temperature reduction, it was phenotypically analyzed by the hematoxylin and eosin (H&E) staining and scanning electron microscopy (SEM). Also, the patterns of differentiation and expression of relevant genes (Sox2, Oct-4, Nanog, TRX, GPX-1, and Sirt1) were assayed by q RT-PCR. Finally, with the help of beta-galactosidase assay the senescence in cell sheets was checked. Results: Data indicated that in the presence of ascorbic acid and selenium, the quality of cell sheet was improved by reducing aged cell numbers. Selenium dramatically produces cell sheets even better than ascorbic acid. The results of both selenium and ascorbic acid were comparable to those of Torolox. Conclusion: Supplementing ascorbic acid and selenium( significantly improved overall properties of generated sheets. The outcomes of this work can be used in optimization andsubsequent medical applications of cell sheets |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| qgbnxvdqyemoywuj.pdf | 1402/08/13 | 170518 | دانلود |
| Abstract book-final-169-170_2.pdf | 1402/08/17 | 228920 | دانلود |
