چاپ صفحه |  صفحه نخست سامانه |  نویسندگان |  اطلاعات تفضیلی |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده ثبت کننده مقاله | عفت علیزاده |
| مرحله جاری مقاله | تایید نهایی |
| دانشکده/مرکز مربوطه | دانشکده علوم نوین پزشکی |
| کد مقاله | 83020 |
| عنوان فارسی مقاله | کاهش مارکرهای پروفیبروتیک در سلولهای ستاره ای کبد از طریق انتقال وابسته به اگزوزوم اوبتیکولیک اسید |
| عنوان لاتین مقاله | Downregulation of profibrotic marker in hepatic stellate cells through Exosome-Mediated Delivery of Obeticholic Acid |
| نوع ارائه | پوستر |
| عنوان کنگره / همایش | پنجمین همایش بینالمللی و سیزدهمین همایش ملی بیوتکنولوژی |
| نوع کنگره / همایش | بین المللی |
| کشور محل برگزاری کنگره/ همایش | Iran (Islamic Republic) |
| شهر محل برگزاری کنگره/ همایش | تهران |
| سال انتشار/ ارائه شمسی | 1402 |
| سال انتشار/ارائه میلادی | 2023 |
| تاریخ شمسی شروع و خاتمه کنگره/همایش | 1402/07/16 الی 1402/07/18 |
| آدرس لینک مقاله/ همایش در شبکه اینترنت | https://biotechcongress.ir/fa/ |
| آدرس علمی (Affiliation) نویسنده متقاضی | Department of Medical Biotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran |
| نویسنده | نفر چندم مقاله |
|---|---|
| آرزو عزیزسلطانی | اول |
| عفت علیزاده | دوم |
| عنوان | متن |
|---|---|
| کلمات کلیدی | : Liver fibrosis, Obeticholic Acid, Hepatic stellate cells, Exosome |
| خلاصه مقاله | Liver fibrosis is associated with the activation of hepatic stellate cells (HSCs) and the upregulation of α-smooth muscle actin (α-SMA) leading to the excessive production and deposition of extra cellular matrix [1, 2]. Aim of this study is to alleviate liver fibrosis by exosome-mediated delivery of obeticholic acid (OCA) to target the activation of HSCs. Following the isolation and validation of exosomes from Wharton's Jelly mesenchymal stem cells (WJ-MSCs), were loaded with OCA using water bath sonication. The MTT assay was employed to determine the cytotoxicity effects of exosomes (Exo) (30µg), OCA-loaded exosomes (OCA-Exo) (~4µM), and free OCA (10µM) on the HSCs (LX2) cell line. Additionally, considering the significant role of HSCs in liver fibrosis progression, the impact of Exo, free OCA, and OCA-Exo on HSC activity was assessed by quantifying the gene and protein expression of α-SMA using qRT-PCR and western blotting. According to MTT assay, the findings revealed that none of the treatments significantly influenced cell viability compared to the control group after 48 hours of treatment. The analysis of mRNA and protein expression demonstrated that the delivery of OCA through Exo led to a more substantial decrease in α-SMA expression compared to the Exo or OCA treatment groups. Based on our study, the combined treatment of Exo-OCA exhibited enhanced effectiveness in targeting HSC activation in hepatic stellate cells compared to exosomes or OCA alone, even at a lower dosage. These results imply a synergistic antifibrotic effect of Exo-OCA in cell culture. |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| 25_0_Arezou Azizsoltani, Effat Alizadeh, Kaveh Baghaei.jpg | 1402/08/10 | 363418 | دانلود |
| Abstract.docx | 1402/08/10 | 17305 | دانلود |
| u6523eedba79ce1.17434577.jpg | 1402/08/10 | 1937427 | دانلود |
