چاپ صفحه |  صفحه نخست سامانه |  نویسندگان |  اطلاعات تفضیلی |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده | نفر چندم مقاله |
|---|---|
| خلیل حاجی اصغرزاده | اول |
| عنوان | متن |
|---|---|
| خلاصه مقاله | Background: Nicotine, as an agonist of nicotinic acetylcholine receptors (nAChRs), binds to these receptors and induces many downstream signaling pathways. Numerous studies have shown that nicotine leads to changes in the expression of microRNAs (miRNAs). Among these miRNAs, miRNA-124 is an essential regulator of gene expression. The present study aimed to investigate the nicotine‑induced effects on miRNA-124 expression in colon cancer cell lines. Methods: First, the SW-480 colon carcinoma cell line was inoculated in 6-well plates to determine the effects of treatments with low and high concentrations of nicotine (1 and 10 μM) on the expression of miRNA-124 and mRNA levels of the α7-subtype of nAChR (α7nAChR) by quantitative real-time PCR. Next, the electroporation method was used to transfer specific siRNA targeting α7nAChR expression after identification of the optimum dose (60 nM) and time period (48 h) of α7nAchR-siRNA. Following that, the changes in the expression of miRNA-124 and α7nAChR were measured after transfection of the α7nAchR-siRNA in combination with nicotine treatment. Results: The findings showed that nicotine increases the expression of both α7nAChR and miRNA-124 in a concentration-dependent manner. Transfection with α7nAChR-siRNA prevents the observed effects of nicotine which indicates that these effects of nicotine are α7nAChR dependent. Conclusions: Nicotine alters miRNA-124 expression in the SW-480 cell line and exerts its effects through the α7nAChR/miRNA-124 axis. Investigating epigenetic changes following nicotine exposure and studying the detailed functional mechanisms of nicotine will lead to finding new therapeutic strategies and prevention of smoking-related diseases. |
| کلمات کلیدی | microRNA-124, Nicotine, Alpha-7 nicotinic acetylcholine receptor, Small interfering RNA, Colorectal cancer |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| PhyPha26-2.pdf | 1402/07/24 | 2214446 | دانلود |
| Abstract 2.pdf | 1402/07/24 | 297933 | دانلود |
