چاپ صفحه |  صفحه نخست سامانه |  نویسندگان |  اطلاعات تفضیلی |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده | نفر چندم مقاله |
|---|---|
| خلیل حاجی اصغرزاده | اول |
| پرویز شهابی | دوم |
| الهام کریمی ثالث | سوم |
| محمدرضا علیپور | چهارم |
| عنوان | متن |
|---|---|
| خلاصه مقاله | Background: Many studies emphasize the role of cigarette smoking and nicotine exposure in increasing liver diseases. However, the detailed mechanisms through which nicotine affects the process of liver fibrosis have not been identified. The present study investigated the effects of nicotine exposure on the modulation of liver fibrosis in an animal model of biliary cirrhosis. Methods: The bile duct ligation model was used to induce cholestatic fibrosis in Wistar rats. The rats were treated with low and high doses of nicotine (1 or 10 mg/kg) for 3 weeks. Then, the spleen-to-body ratio, ductular proliferation, and fibrosis development were evaluated. Also, the mRNA expression of nicotinic acetylcholine receptors and fibrosis genes was measured by real-time PCR. Results: The results showed that nicotine promotes the development of bile duct ligation-induced liver fibrosis. The ratio of spleen/body weight was significantly increased by nicotine exposure. Hematoxylin and eosin and Masson's trichrome staining showed that liver fibrosis has increased in the BDL rats, which was significantly augmented in the nicotine-treated groups. Also, nicotinic acetylcholine receptors and alpha-smooth muscle actin expression were observed in the cholestatic rats and increased after nicotine exposure. The activation of nicotinic acetylcholine receptors triggers biliary proliferation and liver fibrosis. Conclusions: Studying the intracellular mechanism of nicotine and alteration in the expression of nicotinic receptors following nicotine exposure can be useful both in diagnosing nicotine-related diseases and finding new treatment strategies. |
| کلمات کلیدی | Liver fibrosis, Cholestasis, Bile duct ligation, Nicotine, Nicotinic acetylcholine receptors |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| PhyPha26-1.pdf | 1402/07/24 | 2240952 | دانلود |
| Abstract 1.pdf | 1402/07/24 | 261250 | دانلود |
