چاپ صفحه |  صفحه نخست سامانه |  نویسندگان |  اطلاعات تفضیلی |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده | نفر چندم مقاله |
|---|---|
| سهیلا منتظرصاحب | دوم |
| راحله فرحزادی | سوم |
| عنوان | متن |
|---|---|
| خلاصه مقاله | Introduction Natural killer cells (NK cells) are able to kill cancerous cells without prior sensitization. This feature makes them appealing candidates for cellular therapy. Due to the degradation rate and controlled release of these matrices, hydrogels hold great promise in cell differentiation. This study was conducted to elucidate the effect of encapsulated alginate-gelatin on the differentiation potential of C-kit+ cells toward NK cells which are mediated by cytokines detection. Materials and Methods Under both encapsulated and un-encapsulated conditions, C-kit+ cells are capable of differentiating into NK cells. In the following, the cells underwent real-time PCR and western blotting to determine mRNA and protein expression, respectively. A Cytokine antibody array was conducted to determine cytokine profiles from the collected culture medium. The differentiated cells were then co-cultured with Molt-4 cells to examine the expression levels of INF-γ, TNF-α, and IL-10 using real time-PCR. Results There was a substantial change in cytokines levels IL-10 in Molt-4 cells. Conclusions Based on these findings, it can be inferred that the encapsulation effects on differentiation of C-kit+ cells toward NK cells could be related to the secreted cytokines such as interleukin IL-10 |
| کلمات کلیدی | +Cytokine profile, NK cells, C-kit |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| Certificate.jpg | 1402/07/22 | 329054 | دانلود |
| Abstract.jpg | 1402/07/22 | 480670 | دانلود |
