چاپ صفحه |  صفحه نخست سامانه |  نویسندگان |  اطلاعات تفضیلی |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده ثبت کننده مقاله | وحدت پورطهماسبی |
| مرحله جاری مقاله | تایید نهایی |
| دانشکده/مرکز مربوطه | بیماری های عفونی و گرمسیری |
| کد مقاله | 80958 |
| عنوان فارسی مقاله | Comparative RNA-sequencing transcriptome analysis of peripheral blood mononuclear cells in cervical cancer |
| عنوان لاتین مقاله | Comparative RNA-sequencing transcriptome analysis of peripheral blood mononuclear cells in cervical cancer |
| نوع ارائه | سخنرانی |
| عنوان کنگره / همایش | چهاردهمین کنگره بین المللی آزمایشگاه و بالین |
| نوع کنگره / همایش | بین المللی |
| کشور محل برگزاری کنگره/ همایش | Iran (Islamic Republic) |
| شهر محل برگزاری کنگره/ همایش | تهران |
| سال انتشار/ ارائه شمسی | 1401 |
| سال انتشار/ارائه میلادی | 2023 |
| تاریخ شمسی شروع و خاتمه کنگره/همایش | 1401/11/13 الی 1401/11/15 |
| آدرس لینک مقاله/ همایش در شبکه اینترنت | https://isacl2023.congressapp.ir/post_view.php?id=118 |
| آدرس علمی (Affiliation) نویسنده متقاضی | Infectious and Tropical Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran |
| نویسنده | نفر چندم مقاله |
|---|---|
| وحدت پورطهماسبی | اول |
| مریم واعظی | دوم |
| حسین بنازاده باغی | چهارم |
| امید قلیزاده | پنجم |
| نادر محمدزاده | ششم |
| محمد رحمتی | هفتم |
| عنوان | متن |
|---|---|
| کلمات کلیدی | Human papillomavirus, differentially expressed genes, Peripheral blood mononuclear cells, Biomarker |
| خلاصه مقاله | Background: Human papillomavirus (HPV) is a causative agent of cervical cancer, and is major etiologic agent of all the cases. The purpose of this investigation was to use RNAsequencing (RNA-seq) to screen the appropriate differentially expressed genes (DEGs) in the PBMCs for the progressive cervical cancer. Materials and Methods: Here we carried out RNAseq gene expression profiling of 45 peripheral blood mononuclear cells (PBMCs) from Tabriz (northwest of Iran) women, of the disease from cervical intraepithelial neoplasia I (CIN), CINII, CIN-III, healthy control (n= 15), and identified distinct RNA-seq gene expression signatures. The cDNA libraries were paired-end sequenced using an Illumina HiSeq 4000. All raw RNA-sequencing data analyses were performed using conventional RNA-sequencing analysis tools. Next, gene ontology analyses were carried out to elucidate the biological processes of DEGs. Finally, relative transcript abundance of selected DEGs was verified using qRT-PCR on additional validation groups. Results: Specifically, 14, 192, and 895 DEGs were identified for CIN-I, CIN-II and CIN-III, when compared with the healthy subjects. Overall, immune and inflammatory pathways were found to be significantly upregulated in patients with progressive stage. Additionally, we identified candidate signatures of disease progression such as CCL-2, CCL-5, IL-6, IL-10, TNF-α, and ANXA1 among others that were validated by qRTPCR in the samples used for RNA-seq studies as well in an independent set of additional samples. Conclusion: This study provides us with new insights into the biological function and potential cervical cancer and could facilitate the development of diagnostic markers of cervical cancer progression. |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| Oral presentation.JPG | 1401/11/23 | 1226970 | دانلود |
| Oral presentation Abstract.pdf | 1401/11/29 | 779601 | دانلود |
