Electrochemical sensor based on a surface-imprinted polymer- modified electrode for rapid and sensitive detection of S. aureus

Electrochemical sensor based on a surface-imprinted polymer- modified electrode for rapid and sensitive detection of S. aureus


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دانشگاه علوم پزشکی تبریز
دانشگاه علوم پزشکی تبریز

نویسندگان: مسعود مقدس زاده

عنوان کنگره / همایش: 21 امین کنگره بین المللی شیمی , Iran (Islamic Republic) , تبریز , 2022

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نویسنده ثبت کننده مقاله مسعود مقدس زاده
مرحله جاری مقاله تایید نهایی
دانشکده/مرکز مربوطه مرکز تحقیقات بیوتکنولوژی(زیست فناوری)
کد مقاله 80328
عنوان فارسی مقاله Electrochemical sensor based on a surface-imprinted polymer- modified electrode for rapid and sensitive detection of S. aureus
عنوان لاتین مقاله Electrochemical sensor based on a surface-imprinted polymer- modified electrode for rapid and sensitive detection of S. aureus
نوع ارائه پوستر
عنوان کنگره / همایش 21 امین کنگره بین المللی شیمی
نوع کنگره / همایش بین المللی
کشور محل برگزاری کنگره/ همایش Iran (Islamic Republic)
شهر محل برگزاری کنگره/ همایش تبریز
سال انتشار/ ارائه شمسی 1401
سال انتشار/ارائه میلادی 2022
تاریخ شمسی شروع و خاتمه کنگره/همایش 1401/05/04 الی 1401/05/06
آدرس لینک مقاله/ همایش در شبکه اینترنت https://icc21.azaruniv.ac.ir/
آدرس علمی (Affiliation) نویسنده متقاضی مرکز تحقیقات بیوتکنولوژی، دانشگاه علوم پزشکی تبریز، تبریز، ایران

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مسعود مقدس زادهسوم

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عنوان متن
کلمات کلیدیS.aureus, Differential pulse voltammetry, Over-oxided polypyrrole, Glassy carbon electrode, Electrochemical sensor
خلاصه مقالهAccording to the latest statistics, Pathogenic bacteria cause a large number of hospitalizations and deaths worldwide each year. Staphylococcus aureus (S. aureus) is one of the most dangerous zoonotic pathogen responsible for serious bloodstream infections. Therefore, the rapid and sensitive detection and measurement of this bacteria is vital [1-2]. S. aureus-imprinted polymer -based sensor was prepared to detect S. aureus by electropolymerizing pyrrole on the Glassy carbon electrode (GCE) surface using the cyclic voltammetry method in the presence of S. aureus as the template and pyrrole as the functional monomer. After removal the bacterial template from the electrode surface, a series of cavities will be formed in the shape of the desired bacteria in the polymer structure. Then polypyrrole was overoxidized using chronoamperometry method in an alkaline medium [3]. To study the morphology of the desired bacterium, SEM images of different polymer films modifed electrodes were used during the process of bacterial imprinting and recognition. CV and EIS techniques were used to characterize the imprinting process and DPV technique was introduced to draw a calibration graph under optimal conditions and for all of the electrochemical measurements. The selectivity of the proposed sensor was investigated by interfering bacteria. The high selectivity of the sensor against the template bacteria could be ascribed to its sensitivity to the chemical conformation of bacterial outer cell structures. The imprinted electrochemical sensor based on a surface-imprinted polymer- modified electrode was successfully applied to detect S.aureus in food real sample.

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