چاپ صفحه |  صفحه نخست سامانه |  نویسندگان |  اطلاعات تفضیلی |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده ثبت کننده مقاله | وحدت پورطهماسبی |
| مرحله جاری مقاله | تایید نهایی |
| دانشکده/مرکز مربوطه | بیماری های عفونی و گرمسیری |
| کد مقاله | 80300 |
| عنوان فارسی مقاله | Identifying potential gene expression biomarkers in the peripheral blood mononuclear cells ofhepatitis B related hepatocellular carcinoma: a RNA-sequencing-based study |
| عنوان لاتین مقاله | Identifying potential gene expression biomarkers in the peripheral blood mononuclear cells ofhepatitis B related hepatocellular carcinoma: a RNA-sequencing-based study |
| نوع ارائه | سخنرانی |
| عنوان کنگره / همایش | 32nd European Congress of Clinical Microbiology & Infectious Diseases (ECCMID) |
| نوع کنگره / همایش | بین المللی |
| کشور محل برگزاری کنگره/ همایش | Portugal |
| شهر محل برگزاری کنگره/ همایش | Lisbon |
| سال انتشار/ ارائه شمسی | 1401 |
| سال انتشار/ارائه میلادی | 2022 |
| تاریخ شمسی شروع و خاتمه کنگره/همایش | 1401/02/03 الی 1401/02/06 |
| آدرس لینک مقاله/ همایش در شبکه اینترنت | https://markterfolg.de/ESCMID/Final_Programme_2022/#page=1 |
| آدرس علمی (Affiliation) نویسنده متقاضی | Infectious and Tropical Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran |
| نویسنده | نفر چندم مقاله |
|---|---|
| نادر محمدزاده | ششم |
| وحدت پورطهماسبی | اول |
| امید قلیزاده | دهم |
| عنوان | متن |
|---|---|
| کلمات کلیدی | Peripheral blood mononuclear cells ,Hepatocellular carcinoma, RNA-sequencing |
| خلاصه مقاله | Background: Analysis of the gene expression of peripheral blood mononuclear cells (PBMCs) is important to clarify the pathogenesis of hepatocellular carcinoma (HCC) and detection of suitable biomarkers. The purpose of this investigation was to use RNA-sequencing to screen the appropriate differentially expressed genes (DEGs) in the PBMCs for the HCC. Methods: Comprehensive transcriptome of extracted RNA of PBMC (n= 20) from patients with chronic hepatitis B (CHB), liver cirrhosis and early stage of HCC (5 samples per group) was carried out using RNA- sequencing. All raw RNAsequencing data analyses were performed using conventional RNA-seq analysis tools. Next, gene ontology (GO) analyses were carried out to elucidate the biological processes of DEGs. Finally, relative transcript abundance of selected DEGs was 3136 Identifying Potential Gene Expression Biomarkers in the Peripheral Blood Mononuclear Cells of Hepatitis B Related Hepatocellular Carcinoma: A RNAsequencing Based Study verified using quantitative real-time PCR (qRT-PCR) on additional validation groups (n= 100). Hepatitis B surface antigen were sequenced using direct sequencing. Results: Direct sequencing of 681-bp HBsAg indicated that the HBV genotype was D in all of the HBV patients. Specifically, 13, 1262 and 1450 DEGs were identified for CHB, liver cirrhosis, and HCC, when compared with the healthy controls. GO enrichment analysis indicated that HCC is closely related to the immune response. Functional analysis of the hub genes indicated that these genes were mainly involved in immune response and cell proliferation. Seven DEGs (TYMP, TYROBP, CD14, TGFBI, LILRA2, GNLY and GZMB) were common to HCC, cirrhosis and CHB, when compared to healthy controls. The data revealed that the expression of these 7 DEGs were consistent with those from the RNA-sequencing results. Also, expression of 7 representative genes had higher sensitivity were obtained by receiver operating characteristic analysis, which indicated their important diagnostic accuracy for HBV-HCC. Conclusions: This study provide us with new horizons into the biological process and potential prospective clinical diagnosis and prognosis of HCC in the near future. |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| HBV Certificate.pdf | 1401/09/05 | 171111 | دانلود |
| Abstract HBV.pdf | 1401/09/05 | 14610 | دانلود |
| ECCMID Slides.pdf | 1401/10/03 | 1358970 | دانلود |
