چاپ صفحه |  صفحه نخست سامانه |  چکیده مقاله |  نویسندگان |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| To improve the proliferation and differentiation of the dental pulp stem cells (DPSCs), they need to be injected using an accommodating sol-gel hydrogel. In this work, chitosan-based hydrogels were formulated using an optimized preparation strategy using β-glycerophosphate (CS/GP) and genipin (CS/GN) respectively as the ionic and chemical crosslinkers together with sodium hydrogen carbonate as the co-gelling agent. After physicochemical characterization of the two types of hydrogels, the hydrogels were evaluated for their potential in proliferating and/or differentiating dental pulp stem cells (DPSCs) in the presence/absence of mineral trioxide aggregate (MTA) as endodontic filling material and differentiation media (D). The survival and proliferation of DPSCs cultured on the two types of hydrogels were assessed and compared by cell viability and DNA assays. The mineralization, activity of alkaline phosphatase (ALP), and expression of osteo/odontogenic genes of DPSCs grown on the two types of hydrogels were evaluated through alizarin red staining, ALP assay, and real-time PCR, respectively. The findings corroborated that the CS/GN hydrogel can provide better features (e.g., 3D network) than the CS/GP hydrogel. Cytocompatibility and live/dead cell analyses revealed that the DPSCs viability was meaningfully increased using the CS/GN hydrogel. Our findings demonstrated the upregulated osteo/odontogenic expression of DPSCs in the group cultivated using the CS/GN hydrogel supplemented with D + MTA. The calcium deposits and ALP activity were also increased in the DPSCs cultured using CS/GN hydrogel with D + MTA. Collectively, the CS/GN hydrogel along with D + MTA could substantially promote the osteogenic differentiation of DPSCs. |
| نویسنده | نفر چندم مقاله |
|---|---|
| محمد سمیعی | اول |
| الهه دلیرعبدالهی نیا | اول |
| مرضیه فتحی | سوم |
| ژاله برار | چهارم |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| manuscript.pdf | 1401/03/21 | 8271424 | دانلود |
