چاپ صفحه |  صفحه نخست سامانه |  نویسندگان |  اطلاعات تفضیلی |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده ثبت کننده مقاله | اعظم صفری |
| مرحله جاری مقاله | تایید نهایی |
| دانشکده/مرکز مربوطه | بیماری های بافت همبند |
| کد مقاله | 78856 |
| عنوان فارسی مقاله | Designing an Injectable Enzyme-Responsive Chitosan/Gelatin-Based Biosystem in the Treatment of Rheumatoid Arthritis |
| عنوان لاتین مقاله | Designing an Injectable Enzyme-Responsive Chitosan/Gelatin-Based Biosystem in the Treatment of Rheumatoid Arthritis |
| نوع ارائه | پوسترو سخنرانی |
| عنوان کنگره / همایش | پنجمین کنگره بین المللی داروسازی نوین |
| نوع کنگره / همایش | بین المللی |
| کشور محل برگزاری کنگره/ همایش | Iran (Islamic Republic) |
| شهر محل برگزاری کنگره/ همایش | تهران |
| سال انتشار/ ارائه شمسی | 1400 |
| سال انتشار/ارائه میلادی | 2022 |
| تاریخ شمسی شروع و خاتمه کنگره/همایش | 1400/12/24 الی 1400/12/27 |
| آدرس لینک مقاله/ همایش در شبکه اینترنت | https://journals.sbmu.ac.ir/acta/article/view/38044/28641 |
| آدرس علمی (Affiliation) نویسنده متقاضی | Connective Tissue Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran |
| نویسنده | نفر چندم مقاله |
|---|---|
| سید امین شفائی باقری | اول |
| مرضیه فتحی | دوم |
| مصطفی اکبرزاده خیاوی | سوم |
| ام لیلا مولوی | چهارم |
| اعظم صفری | پنجم |
| عنوان | متن |
|---|---|
| کلمات کلیدی | Enzyme-responsive, Hydrogel, Chitosan, Rheumatoid arthritis, Flare-responsive |
| خلاصه مقاله | Rheumatoid arthritis (RA) is one of the most common intricate chronic autoimmune diseases affecting diverse organs and tissues, especially synovial joints. Due to the lack of definite cures for RA by available therapeutic strategies, the goal of medication is to achieve symptomatic relief and limit the advancement of the disease. Drug administration by intraarticular (IA) injection is an emerging popular treatment for RA, which provides localized drugs accumulation and reduces undesirable side effects such as systemic toxicity and gastrointestinal problems. In this regard, biomaterials such as chitosan hydrogels have gained more attention for intra-articular injection because of their perfect biocompatibility and biodegradability, resulting in extended drug retention time and high loading capacity. Besides, cartilage degradation and RA flares are attributed to metalloproteinases (MMPs) enzymes that belong to extracellular matrix metalloproteinase produced by inflamed joint tissues. The objective of the current study was to design an enzyme-responsive drug delivery system based on natural chitosan/gelatin containing diclofenac sodium as a model drug, which could titrate drug release to match the disease activity, resulting in optimal therapeutic efficacy. Chitosan/gelatin hydrogels were prepared using gelatin and chitosan at different -glycerophosphate as an ionic crosslinking agent. Gelation time was determined by the test tube inversion method. The synthesized hydrogels were characterized by FT-IR and scanning electron microscopy. The cytotoxicity of the synthesized hydrogels was investigated on the Huvec cell line by MTT assay. Enzymeresponsive release of diclofenac sodium from prepared hydrogels was investigated in PBS buffer (pH 7.4), with or without collagenase I with incubation at 37 °C and 150 rpm during ten consecutive days. An aliquot of sink medium was removed and replenished with the same volume of fresh PBS at specific time intervals. Drug release was determined by UV-VIS spectrophotometry at 276 nm and the drugs calibration equation. Based on the optimization procedures, the gelation time of the final drug-loaded hydrogel was adjusted to 8 minutes. The cell viability assay of the final formulation revealed that the hydrogels are cytocompatible and exert no/negligible cytotoxicity on Huvec cells. Drug release study showed that the amount of released diclofenac sodium was related to the rate of gelatin degradation, which confirmed the sustained and controlled release in the presence of different concentrations of collagenase. This study develops a novel drug-releasing hydrogel that can effectively respond to arthritis flares. Designed hydrogels can encapsulate a wide range of therapeutic agents, containing enzyme-cleavable sites to facilitate hydrogel disassembly in response to enzymes present in inflammatory environments. The designed hydrogel will be liquid below or at room temperature and indicate fast response thermogelling behavior at the body temperature. |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| Abstract.pdf | 1401/03/18 | 272045 | دانلود |
| 38044-Article Text-171459-1-10-20220404 (1).pdf | 1401/03/18 | 1981564 | دانلود |
