چاپ صفحه |  صفحه نخست سامانه |  نویسندگان |  اطلاعات تفضیلی |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده ثبت کننده مقاله | مرضیه فتحی |
| مرحله جاری مقاله | تایید نهایی |
| دانشکده/مرکز مربوطه | مرکز تحقیقات ریز فناوری دارویی |
| کد مقاله | 78853 |
| عنوان فارسی مقاله | The Effect of Simultaneous Encapsulation of Paclitaxel and Sodium Oxamate in Niosome for the Efficient Treatment of Breast Cancer Cells |
| عنوان لاتین مقاله | The Effect of Simultaneous Encapsulation of Paclitaxel and Sodium Oxamate in Niosome for the Efficient Treatment of Breast Cancer Cells |
| نوع ارائه | سخنرانی |
| عنوان کنگره / همایش | 5th international congress on pharmacy-updates |
| نوع کنگره / همایش | بین المللی |
| کشور محل برگزاری کنگره/ همایش | Iran (Islamic Republic) |
| شهر محل برگزاری کنگره/ همایش | تهران |
| سال انتشار/ ارائه شمسی | 1400 |
| سال انتشار/ارائه میلادی | 2022 |
| تاریخ شمسی شروع و خاتمه کنگره/همایش | 1400/11/26 الی 1400/11/29 |
| آدرس لینک مقاله/ همایش در شبکه اینترنت | https://journals.sbmu.ac.ir/acta/article/view/38044 |
| آدرس علمی (Affiliation) نویسنده متقاضی | Research Center for Pharmaceutical Nanotechnology, Biomedicine Institute, Tabriz University of Medical Sciences, Tabriz, Iran |
| نویسنده | نفر چندم مقاله |
|---|---|
| معصومه کاوه زنجناب | اول |
| الهه دلیرعبدالهی نیا | دوم |
| عفت علیزاده | سوم |
| مرضیه فتحی | چهارم |
| عنوان | متن |
|---|---|
| خلاصه مقاله | Breast cancer is a malignant disease that begins in the breast cells. Paclitaxel disrupts the dynamic balance in microtubules and blocks cells at the end of G2 and M phase of the cell cycle, thus resulting in blocking cell replication. Sodium oxamate is an inhibitor of lactate dehydrogenase (LDH). Because of the low solubility of paclitaxel, nanoparticles (NPs) have been used to overcome this problem. Niosomes are layered vesicles composed of nonionic surfactants that improve therapeutic effects. Different proportions of surfactant 60 and tween 80, PEG 2000 with cholesterol and paclitaxel were added in chloroform and methanol solvents in a round bottom flask and put in a rotary solvent evaporated at approximately 60 ° C and 120 rpm to form a thin layer of surfactant in the flask wall. After 1h, phosphate buffer containing sodium oxamate, hyaluronic acid, and quantum dot was added to the thin layer to prepare the niosomes using the thin film hydration (TFH) method. Nanocarriers size distribution and zeta potential of the prepared niosome were determined by DLS. The amount of loaded drug was measured with a UV spectrophotometer at 230 nm. Release of paclitaxel from niosome was performed at 37 °C with a shaking speed of 150 rpm under various pH values of 5.8 and 7.4. The cytotoxicity of the blank noisome, noisome modified by hyaluronic acid, paclitaxel alone, paclitaxel loaded niosome, and paclitaxel sodium oxamate entrapped in noisome against MCF-7 cell line was investigated via MTT assay in 24, 48, and 72 hours. Abcam mitochondrial staining kit was used in the treated and untreated MCF-7 cells to evaluate the mitochondrial targeting potential of the niosome NPs. The apoptotic effect of the noisome blank, noisome modified by hyaluronic acid, paclitaxel alone, and paclitaxel sodium oxamate loaded in noisome, paclitaxel sodium oxamate-loaded noisome modified by hyaluronic on MCF-7 cell line was evaluated after 72 hours of treatment using flow cytometry to determine the apoptotic effect. The DLS indicated that niosome size was 241 nm with a zeta potential of - 21 mv. The entrapment efficiency of the drug was equal to 80%. Based on the drug release profile, the cumulative drug release was 20% and 80%, respectively at the pH of 7.4 and pH 5.8 after 72 h. Our results have shown that blank niosme and noisome modified by hyaluronic acid did not have toxicity on the breast cancer cells, while paclitaxel-loaded niosome had more fatality than paclitaxel alone. Additionally, sodium oxamate and paclitaxel-loaded niosome increased the mortality of MCF-7 cells compared to paclitaxel-loaded noisome. Moreover, paclitaxel/sodium oxamate-loaded noisome modified by hyaluronic acid had more effects on cancer cells than niosome entrapped paclitaxel/sodium oxamate. These findings were confirmed by results of MTT assay, flow cytometry, and mitochondrial staining kit. |
| کلمات کلیدی | Breast Cancer, Niosome, Paclitaxel, Nanoparticles, Drug delivery, Cytotoxicity |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| Kaveh-2022.pdf | 1401/03/18 | 183120 | دانلود |
| kaveh-certificate.jpeg | 1401/03/18 | 55064 | دانلود |
