چاپ صفحه |  صفحه نخست سامانه |  نویسندگان |  اطلاعات تفضیلی |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده ثبت کننده مقاله | مرضیه فتحی |
| مرحله جاری مقاله | تایید نهایی |
| دانشکده/مرکز مربوطه | مرکز تحقیقات ریز فناوری دارویی |
| کد مقاله | 78852 |
| عنوان فارسی مقاله | Paclitaxel Loaded Chitosan/Copper Sulfide Nanoparticles for Combinational Chemo/Photothermal Therapy of Breast Cancer |
| عنوان لاتین مقاله | Paclitaxel Loaded Chitosan/Copper Sulfide Nanoparticles for Combinational Chemo/Photothermal Therapy of Breast Cancer |
| نوع ارائه | سخنرانی |
| عنوان کنگره / همایش | 5th international congress on pharmacy-updates |
| نوع کنگره / همایش | بین المللی |
| کشور محل برگزاری کنگره/ همایش | Iran (Islamic Republic) |
| شهر محل برگزاری کنگره/ همایش | تهران |
| سال انتشار/ ارائه شمسی | 1400 |
| سال انتشار/ارائه میلادی | 2022 |
| تاریخ شمسی شروع و خاتمه کنگره/همایش | 1400/11/26 الی 1400/11/29 |
| آدرس لینک مقاله/ همایش در شبکه اینترنت | https://journals.sbmu.ac.ir/acta/article/view/38044 |
| آدرس علمی (Affiliation) نویسنده متقاضی | Research Center for Pharmaceutical Nanotechnology, Biomedicine Institute, Tabriz University of Medical Sciences, Tabriz, Iran |
| نویسنده | نفر چندم مقاله |
|---|---|
| شادی بزاززادگان | اول |
| الهه دلیرعبدالهی نیا | دوم |
| خسرو ادیب کیا | سوم |
| مرضیه فتحی | چهارم |
| ژاله برار | پنجم |
| عنوان | متن |
|---|---|
| کلمات کلیدی | Photothermal Therapy, Combination Therapy, Chitosan Coated Copper Sulfide, Nanoparticles, Paclitaxel, Breast Cancer |
| خلاصه مقاله | Photothermal therapy (PTT) is a laser-based, targeted technique for destroying tumor cells that is induced by light-heat conversion in cancer cells until the cells die. PTT has received a lot of attention because of its exceptional specificity, limited invasiveness, and low toxicity to normal tissues, and potent anti-tumor performance. For these reasons, it may be an effective technique for treating kinds of breast cancer that are resistant to conventional therapies. The combination of photothermal therapy and chemotherapy in cancer therapy can lead to the increased therapeutic success of both strategies with lower side effects. For this aim, chitosan-coated copper sulfide nanoparticles (CuS@CS-NPs) were designed and synthesized as a photothermal agent, using copper sulfate as the copper source and sodium sulfide as a sulfide source. Later, nanogels form of nanoparticles (CuS@CS-NGs) were obtained after adding tripolyphosphate pentabasic (TPP). Structural characterization of the prepared nanogels was done by different methods such as FT-IR and XRD analysis. Particle size distribution and zeta potential of the prepared nanogels were determined by DLS. TEM was conducted for morphological analysis. Under the irradiation of 808 nm laser at a power density of 1 W/cm2 the photothermal effects of various concentrations of CuS@CS-NGs solutions were measured. After encapsulation of paclitaxel to CuS@CS-NGs, the amount of loaded drug from nanogels was analyzed using UV absorbance. Release of paclitaxel from nanogels was performed at 37 °C with a shaking speed of 150 rpm under various pH values of 5.8 and 7.4. The cytotoxicity of the nanogels alone and paclitaxel-loaded nanogels against the MCF7 cell line was investigated via MTT assay in presence and absence of NIR irradiation. The apoptotic effect of the nanogels alone and paclitaxel-loaded nanogels on cancer cells was determined after 72 hours of treatment using flow cytometry. After labeling the nanogels alone and paclitaxel-loaded nanogels with FITC, the cellular uptake of nanogels in cancer cells was determined via flow cytometry. TEM images indicated that CuS@CSNPs and CuS@CS-NGs had average size of 10 nm and 30 nm, respectively, while their average hydrodynamic size in aqueous dispersion was 153.7 and 200.9 in the same order which was measured by DLS. Under NIR irradiation, the temperature of an aqueous dispersion containing 5 mg/mL nanogels was dramatically increased from 27 to 55 °C in 360 seconds of exposure, while the temperature elevation of 2 mg/ml nanogel solution was from 27 to 44 °C, which proved that photothermal efficacy could increase with a higher concentration of the photothermal agent. Based on the drug release profile, the cumulative drug release at the pH of 7.4 is 38.99% after 168 h. With lowering the pH of the release medium to 5.8, the drug release reached 35.19%. After introducing paclitaxel to nanogels, the cellular uptake by the cancer cells was increased from 23.05 to 43.02. Based on Flow cytometry-based apoptosis/necrosis assay of MCF-7 cells, we could confirm that a combination of photothermal and chemotherapy has a better cytotoxic effect than each one alone. |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| Shadi-2022.pdf | 1401/03/18 | 217350 | دانلود |
| shadi-certificate.jpeg | 1401/03/18 | 79122 | دانلود |
