چاپ صفحه |  صفحه نخست سامانه |  چکیده مقاله |  نویسندگان |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| Background: The genus Eryngium is a member of the Apiaceae family that has shown diferent pharmacological efects mainly including anti-infammatory, analgesic, anti-cancer, hepatoprotective, and anti-oxidant. Previous research on the anti-cancer activity of Eryngium in some cancer cell lines has led us to explore the anti-proliferative activity of E. caucasicum in the B16F10 cell line. Objective: In this study, the antiproliferative activity of E. caucasicum on melanoma cancer cells (B16F10) and noncancerous cells (HFFF2) were evaluated in vitro. Methods: The dried plant sample of E. caucasicum was extracted by Soxhlet apparatus with n-Hexane, dichloromethane, and methanol solvents. The efects of cytotoxicity of the extracts by the MTT method on melanoma cancer cells (B16F10) and noncancerous cells (HFFF2) was investigated for 24 and 48hours. Then, the cytotoxicity of diferent fractions of the strong extract against normal and cancer cells was evaluated by this method. Annexin V/PI assay was used to study the induction apoptosis via the fractions in cancer cells. Findings: According to the results of the MTT test, n-Hexane extract is the most efective extract against the B16F10 cell line and is a candidate for fractionation with VLC. Among the fractions, 40 and 60% VLC fractions of n-Hexan extract inhibited the growth of B16F10 cells at 24 and 48hours while, these fractions at IC50 concentration had no cytotoxic efects on normal cells. Treatment of cancer cells with efective extract caused apoptosis and necrosis and 40 and 60% more fractions induced apoptosis in these cells. Conclusion: The n-Hexane extract of E. caucasicum and its 40 and 60% fractions showed the highest cytotoxic efect against the B16F10 cell line compared to other extracts and control groups. This inhibition was made through induction of apoptosis. |
| نویسنده | نفر چندم مقاله |
|---|---|
| هاله فروهنده | اول |
| زهرا رضائی پارام | اول |
| ام لیلا مولوی | سوم |
| پرینا اصغریان | چهارم |
| وحیده طرح ریز | پنجم |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| BMC-HF.pdf | 1401/02/29 | 1340035 | دانلود |
