چاپ صفحه |  صفحه نخست سامانه |  چکیده مقاله |  نویسندگان |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| A biocompatible scaffold with appropriate physicochemical properties and efficient biological performance is the main component of bone tissue engineering. Here, we report the fabrication of collagen/P-PCL, a bioactive hybrid scaffold containing collagen and phosphorus-modified polycaprolactone (P-PCL). The phosphorylated polymer was synthesized and characterized by FT-IR spectroscopy. Porous scaffold prepared by mixing and cross-linking collagen and P-PCL solution, followed by freeze-drying method. The obtained hybrid scaffold was characterized by FT-IR and its morphology was observed by SEM. Density, porosity, swelling ratio, mechanical behavior, and degradability of the scaffold were assessed. Finally, biological responses of Adipose-derived Mesenchymal Stem Cells (AD-MSCs) seeded on the scaffold were evaluated. MTT assay revealed that the scaffold could be considered biocompatible. The scaffold was osteoconductive and efficiently support cell attachment and improved proliferation of AD-MSCs. Higher ALP activity and the appearance of more mineralized nodules on the collagen/P-PCL scaffold than in other test groups were concrete proof of the osteoinductivity of this scaffold. Moreover, real-time PCR analyses confirmed that the collagen/P-PCL scaffold was able to induce the AD-MSCs toward osteogenic differentiation. The results suggested that a biodegradable collagen/P-PCL scaffold holds potential for bone regeneration applications. |
| نویسنده | نفر چندم مقاله |
|---|---|
| ایوب آقانژاد | چهارم |
| بنفشه صفری | اول |
| مرضیه آقازاده | دوم |
| لیلا روشنگر | سوم |
| سودابه داوران | پنجم |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| A bioactive porous scaffold containing collagen phosphorous-modified polycaprolactone for osteogenesis of adipose-derived mesenchymal stem cells.pdf | 1401/04/10 | 13214242 | دانلود |
