چاپ صفحه |  صفحه نخست سامانه |  نویسندگان |  اطلاعات تفضیلی |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده | نفر چندم مقاله |
|---|---|
| امیرمحمد شرفی | دوم |
| عنوان | متن |
|---|---|
| خلاصه مقاله | L-asparaginase enzyme is known to be a fundament treatment of acute lymphoblastic leukemia, and also the efficient factor in decreasing of acrylamide formation in food industry. Generally, prokaryotic source of L-asparaginase enzymes is used as a medicinal purpose in ALL patients. The main obstacle in ALL treatment with bacterial L-asparaginase is undesirable side effects including immunological hypersensitivity reactions. Therefore, other source of L-asparaginase is subject of new research. Candida Utilis is capable of producing L-asparaginase as a eukaryotic source. To enhance production of L-asparaginase by Candida Utilis first sequencing batch reactor (SBR) were used to produce higher concentration of L-asparaginase. Consequences are as follows, firstly production of L-asparaginase from Candida Utilis at SBR was leaded in increasing of L-asparaginase activity from 4.8 IU/ml to 15.31IU/ml in the first and ninth batch. Secondly, period of batches was decreased during SBR from 22h to 4h, respectively. Ultimately cell protein concentration was increased from 1117mg/l to 2120mg/l. As well achieving to the species of Candida Utilis being morphologically different from primary cells had been another significant advantage of SBR while no genetic modification was performed. Next optimization of culture conditions for L-asparaginase production by submerged fermentation of Candida Utilis was studied which I modeled in RSM in order to reduce the number of experiments. The experimental L-asparaginase activity of 36 IU/ml was obtained at the optimum conditions of molasses and corn steep liquor, pepton as carbon and nitrogen source. |
| کلمات کلیدی | Keyword: L-asparaginase, Acute lymphoblastic leukemia, Sequencing Batch Reactor, Candida Utilis, optimization. |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| international congress of biotechnology - certificate of oral presentation.jpg | 1401/01/24 | 198248 | دانلود |
| abstract - international congress of biotechnology - booklet-2.pdf | 1401/01/24 | 1480851 | دانلود |
| 1.docx | 1401/01/24 | 16299 | دانلود |
