چاپ صفحه |  صفحه نخست سامانه |  چکیده مقاله |  نویسندگان |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| The glycan receptor is a glycosylphosphatidylinositol glycoprotein that is overexpressed on the surface of various cancer cells and has been utilized for wide applications. In the present work, the surface of citratecapped gold nanoparticles (cit-AuNPs) was modified with mercaptopropionic acid (MPA) molecules to provide carboxylic groups for secondary functionalization with amine anchored-silica quantum dots (SiNH2 QDs) to produce cit-AuNPs-MPA/Si-NH2 QDs fluorescent nanoparticles. Concanavalin A (Con A) molecules were attached through thiol–AuNP bonds to produce the final cit-AuNPs/MPA/Si-NH2 QDs/ Con A smart nanoparticles. The synthesized novel cit-AuNPs/MPA/Si-NH2 QDs/Con A nanoparticles were utilized for the bioimaging of glycan-overexpressed breast cancer cells. Fluorescence microscopy and flow cytometry results revealed that the cit-AuNPs/MPA/Si-NH2 QDs/Con A NPs can be efficiently taken up by cancer cells, with differentiating ability between overexpressed cancer cells and lowexpressed normal cells. The cellular viability of the cit-AuNPs/MPA/Si-NH2 QDs/Con A NPs was tested by the MTT test, proving their biocompatible nature at the 200 mg mL1 level. In conclusion, the fabricated cit-AuNPs/MPA/Si-NH2 QDs/Con A NPs could be utilized for the bioimaging of MCF-7 cancer cells even in the clinical setting after proper in vivo validation. |
| نویسنده | نفر چندم مقاله |
|---|---|
| حسن باقرپور شاملو | سوم |
| نسرین بارگاهی | دوم |
| جعفر سلیمانی | چهارم |
| سمیه جعفرزاده | اول |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| d2ra00035k.pdf | 1400/12/28 | 1295252 | دانلود |
