چاپ صفحه |  صفحه نخست سامانه |  چکیده مقاله |  نویسندگان |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| Background: Recently, melatonin has attracted massive attention due to its anticancer effect on various human malignancies. It has also been demonstrated that melatonin is useful in combating resistance against conventional chemotherapeutics. Objectives: This study aimed to evaluate melatonin’s effects on multidrug resistance (MDR) in human myelogenous leukemia cells. Methods:Melatonin’s cytotoxicity on the K562 and K562/doxorubicin (DOX) cell lines was assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The expression and activity of P-glycoprotein (P-gp) were measured as well. The mRNA and protein expression levels of tensin homolog deleted on chromosome ten (PTEN) was assessed in cells. Eventually, apoptosisin cancer cells was measured through Annexin V/PI staining. Results:Treatment with melatonin significantly increased the cytotoxicity of DOX in resistant K562 cells. The expression and activityof P-gp were attenuated following melatonin treatment. In addition, melatonin upregulated PTEN in K562/DOX cells. Melatonin also augmented apoptosisin combination with DOX. Conclusion:Melatonin effectively increased the cytotoxic effects of DOX in K562/DOX cells through down-regulation of P-gp and up-regulation of PTEN in resistant K562 cells. |
| نویسنده | نفر چندم مقاله |
|---|---|
| بهمن یوسفی | پنجم |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| 212-Article Text-7883-9101-10-20211021.pdf | 1400/11/30 | 857236 | دانلود |
