چاپ صفحه |  صفحه نخست سامانه |  نویسندگان |  اطلاعات تفضیلی |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده ثبت کننده مقاله | وحدت پورطهماسبی |
| مرحله جاری مقاله | تایید نهایی |
| دانشکده/مرکز مربوطه | بیماری های عفونی و گرمسیری |
| کد مقاله | 78086 |
| عنوان فارسی مقاله | Detection and quantification of HBV genomes in PBMC of chronic HBV infection, cirrhosis and hepatocellular carcinoma patients |
| عنوان لاتین مقاله | Detection and quantification of HBV genomes in PBMC of chronic HBV infection, cirrhosis and hepatocellular carcinoma patients |
| نوع ارائه | پوستر |
| عنوان کنگره / همایش | 13th International Congress of Medical Laboratory and Clinic |
| نوع کنگره / همایش | بین المللی |
| کشور محل برگزاری کنگره/ همایش | Iran (Islamic Republic) |
| شهر محل برگزاری کنگره/ همایش | تهران |
| سال انتشار/ ارائه شمسی | 1400 |
| سال انتشار/ارائه میلادی | 2022 |
| تاریخ شمسی شروع و خاتمه کنگره/همایش | 1400/11/16 الی 1400/11/20 |
| آدرس لینک مقاله/ همایش در شبکه اینترنت | https://congressapp.ir/isacl2022/poster_view.php?id=800 |
| آدرس علمی (Affiliation) نویسنده متقاضی | Infectious and Tropical Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran |
| نویسنده | نفر چندم مقاله |
|---|---|
| آرزو آزادی | چهارم |
| وحدت پورطهماسبی | اول |
| عنوان | متن |
|---|---|
| خلاصه مقاله | Background and Aim: Increasing study revealed that the hepatitis B virus (HBV) exist in peripheral blood mononuclear cells (PBMCs). It remains poorly understood whether HBV DNA and covalently closed circular DNA (cccDNA) can be emerged in the PBMC of patients with different stage of HBV infection. The aim of this study was to compare the detection of HBV DNA and quantification and presence of cccDNA within PBMC from chronic HBV infection (CHB), cirrhosis and hepatocellular carcinoma (HCC) individuals. Methods: Present study has been conducted on 120 participants (30 CHB patients, 30 cirrhosis patients, 30 HCC patients and 30 healthy control) from Tehran, Iran. HBV serological markers were tested by ELISA assay. PBMC of all individuals were assayed for HBV DNA detection and quantification and presence of cccDNA. Results: Of all 90 HBV patients, 58 (64.4%) were positive for HBV DNA in PBMC. HBV DNA was detected in PBMC isolated from 13/30 CHB patients, 20/30 cirrhosis patients and 25/30 HCC patients. Also, 6 (20%) of CHB, 13 (43.3%) of cirrhosis, and 16 (15.3%) of HCC patients were cccDNA positive. The serum HBV viral loads were statistically higher than the HBV viral loads of PBMCs (P-value< 0.001. Positive correlation was found between HBV DNA loads in serum and PBMC of patients. Moreover, HBV DNA quantity of serum and PBMC in terms of HBeAg status showed considerable association. Conclusion: HBV quantity in PBMC correlated with serum HBV viral load. HBV genomes in PBMC may be a risk factor for HBV disease progression. |
| کلمات کلیدی | : Hepatitis B virus; Chronic hepatitis B; Cirrhosis; Hepatocellular carcinoma; peripheral blood mononuclear cells; Covalently closed circular DNA |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| 13th ISACL Abstract Book-2.pdf | 1400/11/23 | 5527619 | دانلود |
| HBV PBMC Vahdat Poortahmasebi.jpg | 1400/11/23 | 382858 | دانلود |
