چاپ صفحه |  صفحه نخست سامانه |  چکیده مقاله |  نویسندگان |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| Objective: Amnion membrane (AM) has been popular for the treatment of inflammatory disorders due to its cell repairing properties. This current study aims to find the underlying mechanisms of amnion membrane proteins (AMPs) against the pro-inflammatory miRNA, miR-155, miR-146, and antiapoptotic microRNA, miR-21, in LPS-treated H9c2 cells. Methods: Cell viability and apoptosis were determined by MTT assay and annexin V/PI staining. The production of the cytokines, TNF-a and IL-6 were evaluated by using qPCR and Enzyme-linked immunosorbent assay (ELISA), respectively. In addition, the expression of miRNAs was quantified by qPCR, and also the protein level of TLR4 and NF-kb was determined with western blotting. Results: We found that AMPs ameliorated LPS-induced reduction of cell viability and augment apoptosis in H9c2 cells. AMPs efficiently inhibited cytokine expression (IL-6 and TNF-a) and activity of TLR4/ NF-jB pathway in LPS-treated H9c2 cells. Correspondingly, in parallel with the suppression of proinflammatory cytokines and apoptosis, AMPs mitigated pro-inflammatory miRNA, miR-155 expression, while, the expression of miR-155 was found to be increased in LPS-treated H9c2 cells. Also, AMPs activated miR-146 expression in H9c2 cells under LPS treatment. Additionally, the elevated expression of miR-21 provoked by LPS was further enhanced by AMPs. Conclusions: In conclusion, AMPs could alleviate LPS-induced cardiomyocytes cells injury via up-regulation of miR-21, miR-146, and suppression of TLR4/NF-jB pathway, which plays a key role in the down-regulation of LPS-mediated miR-155 and inflammatory cytokine expressio |
| نویسنده | نفر چندم مقاله |
|---|---|
| آرزو رضائی نژاد زمانی | چهارم |
| ناصر صفائی آغبلاغ | نهم |
| یوسف فریدوند | دهم |
| داود قلیزاده | هشتم |
| محمد نوری | هفتم |
| مسعود پزشکیان | ششم |
| احمد رضا جودتی | پنجم |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| Amnion membrane proteins H9c2.pdf | 1400/11/05 | 1805564 | دانلود |
