چاپ صفحه |  صفحه نخست سامانه |  چکیده مقاله |  نویسندگان |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| α-Amylase encapsulated in water in oil (W/O) emulsion was prepared using poly ethylene glycol (PEG 10000) (2%w/v) as water phase and ethylcellulose (EC) in ethyl acetate as oil phase at the ratio of 10:90 v/v. Next, the electrospun fibers were prepared by mixing enzyme loaded emulsion with EC solution (20%w/v) in acetic acid/ethyl acetate (20:80 v/v) at the 2:1 ratio. The emulsion showed good physical stability. The immobilized enzyme showed high activity across a board range of pHs and temperatures. The storage stability of the immobilized enzyme was 2 fold of free enzyme activity after 45 days. The residual activity of immobilized α–amylase onto of fibers after 10 and 15 repeated cycles, was approximately 100% and 50%, respectively. The results of this study indicated that the α–amylase loaded EC fibers have acceptable activity against harsh conditions and excellent reusability. |
| نویسنده | نفر چندم مقاله |
|---|---|
| سیداسدالله حسینی | اول |
| مهناز طبیبی اذر | سوم |
| مریم محمدی | چهارم |
| زهرا گلچین فر | پنجم |
| مریم محمودزاده مغانجوقی | ششم |
| علی جهانبان اسفهلان | هفتم |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| immoblization.pdf | 1400/10/13 | 1942187 | دانلود |
