| Antibiotic resistance is one of the serious health-threatening issues globally, the control
of which is indispensable for rapid diagnosis and treatment because of the high prevalence
and risks of pathogenicity. Traditional and molecular techniques are relatively expensive,
complex, and non-portable, requiring facilities, trained personnel, and high-tech laboratories.
Widespread and timely-detection is vital to the better crisis management of rapidly spreading
infective diseases, especially in low-tech regions and resource-limited settings. Hence, the need
for inexpensive, fast, simple, mobile, and accessible point-of-care (POC) diagnostics is highly
demanding. Among different biosensing methods, the isothermal amplification of nucleic acids
is favorite due to their simplicity, high sensitivity/specificity, rapidity, and portability, all because
they require a constant temperature to work. Isothermal amplification methods are utilized
for detecting various targets, including DNA, RNA, cells, proteins, small molecules, ions, and
viruses. In this paper, we discuss various platforms, applications, and potentials of isothermal
amplification techniques for biosensing of antimicrobial resistance. We also evaluate the
potential of these methods, coupled with the novel and rapidly-evolving platforms offered by
nanotechnology and microfluidic devices. |