چاپ صفحه |  صفحه نخست سامانه |  نویسندگان |  اطلاعات تفضیلی |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده | نفر چندم مقاله |
|---|---|
| مریم زین العابدینی | اول |
| مسعود مقدس زاده | دوم |
| عنوان | متن |
|---|---|
| خلاصه مقاله | Background Influenza is as an extremely contagious respiratory tract disease. Influenza A virus is a member of the Orthomyxoviridae family that has segmented genome and great antigenic diversity. Influenza viruses possess multiple conserved epitopes that are designed for universal vaccines. These epitopes are the nucleoprotein (NP) and HA2. Methods In this survey, we have constructed new plasmids to express above-mentioned epitopes as a single recombinant product. To this aim, two peptide genes of N-terminal NP and HA2 were linked together with a (Gly4Ser) 4 peptide linker, synthesized, and cloned into pET26b vector. Then, the construct was transferred into E. coli BL21 (DE3) cells and induced using isopropyl β-D-1 thiogalactoside (IPTG). Immunization of mice with above peptides significantly induced humoral immune responses. Three weeks after the last booster, mice were inoculated intranasally with 1×106 EID50 of H9N2 virus. Results The recombinant NP-HA2 fusion protein gene was cloned into pET26b vector. The results of sequencing displayed that gene was properly cloned in vector. Also, SDS-PAGE showed a strong single band. Moreover, Western blot analysis indicated a single band in correct position. Real-time RT-PCR studies exhibited reduction of virus in BALB/c mice lung tissues. Our study revealed that this protein could protect mice against H9N2 virus. Conclusions The recombinant NP-HA2 fusion protein characterizes a potential candidate for influenza vaccine studies in animal model. According to the findings, the NP-HA2 fusion protein induced humoral immunity responses. The findings of this study suggest that the recombinant fusion peptide which is economical to yield can be used for induction specific antibodies responses. The results of the current study showed that above protein can protect mice by decreasing virus shedding. |
| کلمات کلیدی | NP-HA2 |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| Final Programme of ECCMID 2021.pdf | 1400/09/06 | 15031029 | دانلود |
| Acceptance.jpg | 1400/09/06 | 189397 | دانلود |
| Protection against H9N2 Influenza A virus induced by recombinant NP-HA2 fusion protein (1).pdf | 1400/09/08 | 130815 | دانلود |
